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Rapid detection of mutations and chromosomal rearrangements in T-DNA insertion mutants of Colletotrichum higginsianum using long-read whole-genome sequencing
Random mutagenesis is a useful tool for identifying genes of interest. Here, we used long-read whole-genome sequencing analysis to identify T-DNA...
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Unveiling microbial diversity: harnessing long-read sequencing technology
Long-read sequencing has recently transformed metagenomics, enhancing strain-level pathogen characterization, enabling accurate and complete...
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Genome analysis of Zoysia japonica ‘Yaji’ cultivar using PacBio long-read sequencing
The Zoysia japonica plant is a warm-season turfgrass and a horticultural crop used in various places and an important genetic resource because it is...
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Long-read sequencing reveals the structural complexity of genomic integration of HPV DNA in cervical cancer cell lines
BackgroundCervical cancer (CC) causes more than 311,000 deaths annually worldwide. The integration of human papillomavirus (HPV) is a crucial genetic...
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A survey of algorithms for the detection of genomic structural variants from long-read sequencing data
As long-read sequencing technologies are becoming increasingly popular, a number of methods have been developed for the discovery and analysis of...
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Long-read sequencing of the zebrafish genome reorganizes genomic architecture
BackgroundNanopore sequencing technology has revolutionized the field of genome biology with its ability to generate extra-long reads that can...
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DNA Isolation Long-Read Genomic Sequencing in Ctenophores
Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining...
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A comparison of methods for detecting DNA methylation from long-read sequencing of human genomes
BackgroundLong-read sequencing can enable the detection of base modifications, such as CpG methylation, in single molecules of DNA. The most commonly...
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Caveats for Watermelon Whole Genome Sequencing
Watermelon is a highly important vegetable crop species worldwide, with its abundance of genetic resources aiding in identifying mechanisms and...
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Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflows
BackgroundLong read sequencing allows the analysis of full-length transcripts in plants without the challenges of reliable transcriptome assembly....
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Analyzing somatic mutations by single-cell whole-genome sequencing
Somatic mutations are the cause of cancer and have been implicated in other, noncancerous diseases and aging. While clonally expanded mutations can...
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Comparative analysis of metagenomic classifiers for long-read sequencing datasets
BackgroundLong reads have gained popularity in the analysis of metagenomics data. Therefore, we comprehensively assessed metagenomics classification...
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Whole-genome sequencing in medicinal plants: current progress and prospect
Advancements in genomics have dramatically accelerated the research on medicinal plants, and the development of herbgenomics has promoted the...
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Assembling Plant Genomes with Long-Read Sequencing
Continuous improvements in long-read sequencing allow us to tackle increasingly big and complex genomes. Here we present the principles of long-read...
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Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing
The plant microbiome is crucial for plant growth, yet many important questions remain, such as the identification of specific bacterial species in...
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In it for the long run: perspectives on exploiting long-read sequencing in livestock for population scale studies of structural variants
Studies have demonstrated that structural variants (SV) play a substantial role in the evolution of species and have an impact on Mendelian traits in...
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Accurate Sequencing and Haplotyping from 10 Cells Using Long Fragment Read (LFR) Technology
In this chapter, we describe how Long Fragment Read (LFR) technology can be applied to samples consisting of very few cells (5–20) to enable complete...
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Unveiling novel genetic variants in 370 challenging medically relevant genes using the long read sequencing data of 41 samples from 19 global populations
Background: A large number of challenging medically relevant genes (CMRGs) are situated in complex or highly repetitive regions of the human genome,...
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Benchmarking long-read RNA-sequencing analysis tools using in silico mixtures
The lack of benchmark data sets with inbuilt ground-truth makes it challenging to compare the performance of existing long-read isoform detection and...
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Nanopore-Based Long-Read Sequencing Technology to Obtain Highly Contiguous Whole-Genome Sequence of Actinobacterial Genomes like Streptomyces Sp.: A Complete Guide for Actinobacterial Whole Genome Sequencing Project Using Nanopore
The “postgenomic era” will be dominated by long-read technologies like Oxford Nanopore technology and Pacific Biosystem’s single-molecule real-time...
