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Ctenophores

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Leonid L. Moroz

    1. Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2757)

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About this book

This volume explores emerging approaches and methods inspired by recent research using ctenophores or placozoans, facilitating the study of these enigmatic species and ancient animal lineages. Practical protocols are integrated with introductory chapters about general biology, summarizing the diversity, evolution, genomics, development, and neurobiology of these organisms. Special chapters outline strategies and applications for culturing, microscopy, physiology, electrical synapses, and bioluminescence, molecular and single-cell biology, transcription factors, as well as gene and protein expression analyses. The book also contains bioinformatics and computational chapters covering peptidomics and epigenomics. Written for the highly successful Methods in Molecular Biology series, chapters include detailed implementation advice that leads to dependable results in the laboratory. 

Authoritative and practical, Ctenophores: Methods and Protocols is anideal resource for comparative and experimental biologists and interdisciplinary scientists aiming to decipher fundamental principles of animal organization, origin, and the evolution of biological complexity in general, opening novel avenues for future synthetic biology research.

Keywords

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Brief History of Ctenophora

    • Leonid L. Moroz
    Pages 1-26

  3. Ctenophora: Illustrated Guide and Taxonomy

    • Leonid L. Moroz, Richard Collins, Gustav Paulay
    Pages 27-102

  4. Brief History of Placozoa

    • Daria Y. Romanova, Leonid L. Moroz
    Pages 103-122

  5. Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi

    • Joan J. Soto-Angel, Eva-Lena Nordmann, Daniela Sturm, Maria Sachkova, Kevin Pang, Pawel Burkhardt
    Pages 123-145

  6. Illustrated Neuroanatomy of Ctenophores: Immunohistochemistry

    • Tigran P. Norekian, Leonid L. Moroz
    Pages 147-161

  7. Scanning Electron Microscopy of Ctenophores: Illustrative Atlas

    • Tigran P. Norekian, Leonid L. Moroz
    Pages 163-184

  8. DNA Isolation Long-Read Genomic Sequencing in Ctenophores

    • David Moraga Amador, Andrea B. Kohn, Yelena Bobkova, Nedka G. Panayotova, Leonid L. Moroz
    Pages 185-200

  9. RNA Isolation from Ctenophores

    • Andrea B. Kohn, Yelena Bobkova, Leonid L. Moroz
    Pages 201-214

  10. Gene Expression Patterns in the Ctenophore Pleurobrachia bachei: In Situ Hybridization

    • Andrea B. Kohn, Yelena Bobkova, Leonid L. Moroz
    Pages 215-237

  11. Characterization of the Mitochondrial Proteome in the Ctenophore Mnemiopsis leidyi Using MitoPredictor

    • Viraj Muthye, Dennis V. Lavrov
    Pages 239-257

  12. Expression and Functional Analysis of Ctenophore Glutamate Receptor Genes

    • Mark L. Mayer
    Pages 259-268

  13. Expression, Purification, and Determination of Sensitivity to Calcium Ions of Ctenophore Photoproteins

    • Lyudmila P. Burakova, Svetlana V. Markova, Natalia P. Malikova, Eugene S. Vysotski
    Pages 269-287

  14. Functional Screening of cDNA Expression Library for Novel Ctenophore Photoproteins

    • Svetlana V. Markova, Eugene S. Vysotski
    Pages 289-306

  15. Recording Cilia Activity in Ctenophores

    • Tigran P. Norekian, Leonid L. Moroz
    Pages 307-313

  16. Electrophysiology of Ctenophore Smooth Muscle

    • Robert W. Meech, André Bilbaut (Deceased), Mari-Luz Hernandez-Nicaise
    Pages 315-359

  17. Gap Junctions in Ctenophora

    • Andrea B. Kohn, Leonid L. Moroz
    Pages 361-381

  18. Analysis and Visualization of Single-Cell Sequencing Data with Scanpy and MetaCell: A Tutorial

    • Yanjun Li, Chaoyue Sun, Daria Y. Romanova, Dapeng O. Wu, Ruogu Fang, Leonid L. Moroz
    Pages 383-445

  19. DNA Methylation in Ctenophores

    • Emily C. Dabe, Andrea B. Kohn, Leonid L. Moroz
    Pages 447-460

  20. Ocean to Tree: Leveraging Single-Molecule RNA-Seq to Repair Genome Gene Models and Improve Phylogenomic Analysis of Gene and Species Evolution

    • Jan Hsiao, Lola Chenxi Deng, Leonid L. Moroz, Sreekanth H. Chalasani, Eric Edsinger
    Pages 461-490
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Editors and Affiliations

  • Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, USA

    Leonid L. Moroz

Bibliographic Information

  • Book Title: Ctenophores

  • Book Subtitle: Methods and Protocols

  • Editors: Leonid L. Moroz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3642-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3641-1Published: 27 April 2024

  • Softcover ISBN: 978-1-0716-3644-2Due: 11 May 2025

  • eBook ISBN: 978-1-0716-3642-8Published: 26 April 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 585

  • Number of Illustrations: 36 b/w illustrations, 218 illustrations in colour

  • Topics: Biological Techniques

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