Abstract
Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora. Specifically, we describe a Pacific Biosciences library construction method used in conjunction with gDNA isolation methods that have proven successful in obtaining high-quality genomic assemblies in ctenophores.
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Acknowledgments
This work was supported in part by the Human Frontiers Science Program (RGP0060/2017) and the National Science Foundation (IOS-1557923) grants to L.L.M. Research reported in this publication was also supported in part by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award number R01NS114491 (to L.L.M). The content is solely the authors’ responsibility and does not necessarily represent the official views of the National Institutes of Health.
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Moraga Amador, D., Kohn, A.B., Bobkova, Y., Panayotova, N.G., Moroz, L.L. (2024). DNA Isolation Long-Read Genomic Sequencing in Ctenophores. In: Moroz, L.L. (eds) Ctenophores. Methods in Molecular Biology, vol 2757. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3642-8_7
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DOI: https://doi.org/10.1007/978-1-0716-3642-8_7
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