This Registered Report presents the results of the Long-read RNA-Seq Genome Annotation Assessment Project, which is a community effort for benchmarking long-read methods for transcriptome analyses, including transcript isoform detection, quantification and de novo transcript detection.

Assessing forest health and resilience is an urgent, many-methods task that takes global collaboration, data collected at multiple scales and a willingness to be surprised.

Tissues and organs are inherently three-dimensional. Studies to understand their function and dysfunction should therefore aim to maintain the 3D spatial context.

Bats, the only flying mammals, comprise almost 25% of mammalian species. They are excellent navigators, highly social, and extremely long-lived. Their sense of echolocation has been studied for many years — but many species possess also excellent vision and olfaction. In recent years, bats have emerged as new models for neurobiology of navigation, social neuroscience, aging, and immunity.

EpiChem enables the co-profiling of small molecule–DNA interactions and multiple epigenomic features in single cells.

cryoDRGN-ET is a generative neural network method for heterogeneous reconstruction of cryo-ET subtomograms. Using subtomogram tilt-series images, it can capture states diverse in both composition and conformation.

Onavg is a surface template of the human cortex. In contrast to existing templates, the cortical surface is uniformly sampled, which has advantages in numerous applications.

SwitchSeeker combines computational and experimental techniques to identify functional RNA structural switches. Applied to the human transcriptome, it identified a novel RNA switch in the 3ʹUTR of RORC, linked to nonsense-mediated decay.

Keypoint-MoSeq is an unsupervised behavior segmentation algorithm that extracts behavioral modules from keypoint tracking data acquired with diverse algorithms, as demonstrated in mice, rats and fruit flies. The extracted modules faithfully reflect human-annotated behaviors even though they are obtained in an unsupervised fashion.

This work presents ORFtag that enables proteome-wide functional screens by tagging and overexpression of endogenously encoded proteins via randomly integrated cassettes.

Being first in a family to aim for a science career takes resilience and resourcefulness.

Bats, the only flying mammals, comprise almost 25% of mammalian species. They are excellent navigators, highly social, and extremely long-lived. Their sense of echolocation has been studied for many years — but many species possess also excellent vision and olfaction. In recent years, bats have emerged as new models for neurobiology of navigation, social neuroscience, aging, and immunity.

Tissues and organs are inherently three-dimensional. Studies to understand their function and dysfunction should therefore aim to maintain the 3D spatial context.

Machine learning approaches can distinguish six different classes of presynapses from electron micrographs across the Drosophila brain.