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Plant Gametogenesis

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Christophe Lambing

    1. Department of Plant Sciences, University of Cambridge, Cambridge, UK

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2484)

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About this book

This volume provides protocols and methods on techniques to study plant gametogenesis. Chapters are divided into four sections covering omics, cytological, molecular approaches, plant transformation, genome editing, bioinformatics, and data analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Plant Gametogenesis: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Omics Approaches to Study Male and Female Germlines in Plants

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry

      • Monica Borghi, Leonardo Perez de Souza, Alisdair R. Fernie
      Pages 3-12

    3. Proteomic-, Phosphoproteomic-, and Acetylomic-Based Mass Spectrometry to Identify Tissue-Specific Protein Complexes and Phosphorylation in Plant Gametogenesis

      • Lu Sun, Aowei Mo, Pingli Lu
      Pages 13-22

    4. Identifying small RNAs and Analyzing Their Association with Gene Expression Using Isolated  Arabidopsis Male Meiocytes

      • Jiyue Huang, Chenjiang You, Cong Wang, Yingxiang Wang, Gregory P. Copenhaver
      Pages 23-41

    5. CTAB DNA Extraction and Genotyping-by-Sequencing to Map Meiotic Crossovers in Plants

      • Pallas Kuo, Ian R. Henderson, Christophe Lambing
      Pages 43-53

    6. Interrogating Global Chromatin Interaction Network by High-Throughput Chromosome Conformation Capture (Hi-C) in Plants

      • Wenjing Wang, Longjian Niu, Chunhui Hou
      Pages 55-67

  3. Microscopy Approaches to Study Gametogenesis in Plants

    1. Front Matter

      Pages 69-69
      Download chapter PDF

    2. Cytogenetic Techniques for Analyzing Meiosis in Hexaploid Bread Wheat

      • Kim Osman, F. Chris H. Franklin, Eugenio Sanchez-Moran
      Pages 71-84

    3. Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum

      • Pallas Kuo, Alice Darbyshire, Christophe Lambing
      Pages 85-92

    4. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

      • Sona Valuchova, Pavlina Mikulkova, Jana Pecinkova, Karel Riha
      Pages 93-105

    5. Bimolecular Fluorescence Complementation to Test for Protein–Protein Interactions and to Uncover Regulatory Mechanisms During Gametogenesis

      • Ramakrishna Yadala, Maryia Ratnikava, Inna Lermontova
      Pages 107-120

    6. Quantifying Meiotic Crossover Recombination in Arabidopsis Lines Expressing Fluorescent Reporters in Seeds Using SeedScoring Pipeline for CellProfiler

      • Nadia Kbiri, Julia Dluzewska, Ian R. Henderson, Piotr A. Ziolkowski
      Pages 121-134

  4. Plant Manipulation and Genome Editing

    1. Front Matter

      Pages 135-135
      Download chapter PDF

    2. Generation of Mutants by Combined Treatment of Physical and Chemical Mutagens in Rice

      • Yuanyuan Tan, Shan Li
      Pages 137-142

    3. Generation of Mutant Plants by Gamma Ray Exposure and Development of Low-Cost TILLING Population in Chickpea (Cicer arietinum L.)

      • Wahiba Amri-Tiliouine, Joanna Jankowicz-Cieslak, Bradley J. Till, Meriem Laouar
      Pages 143-159

    4. Efficient Generation of CRISPR /Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions

      • Tomasz Bieluszewski, Maja Szymanska-Lejman, Wojciech Dziegielewski, Longfei Zhu, Piotr A. Ziolkowski
      Pages 161-182

    5. Generation of Deletion Lines in Allohexaploid Bread Wheat

      • Heïdi Serra, Radim Svačina, Jan Bartoš, Pierre Sourdille
      Pages 183-199

    6. Post-transcriptional Gene Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in Tomato

      • Zhengming Wang, Xiao Yu Wang, Claudia Martinho, David C. Baulcombe
      Pages 201-212

    7. Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling

      • Siddique I. Aboobucker, Talukder Z. Jubery, Ursula K. Frei, Yu-Ru Chen, Tyler Foster, Baskar Ganapathysubramanian et al.
      Pages 213-235
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Editors and Affiliations

  • Department of Plant Sciences, University of Cambridge, Cambridge, UK

    Christophe Lambing

Bibliographic Information

  • Book Title: Plant Gametogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Christophe Lambing

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2253-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2252-0Published: 24 April 2022

  • Softcover ISBN: 978-1-0716-2255-1Published: 24 April 2023

  • eBook ISBN: 978-1-0716-2253-7Published: 23 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 382

  • Number of Illustrations: 10 b/w illustrations, 83 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Bioinformatics

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