Abstract
Random mutagenesis is a useful tool for identifying genes of interest. Here, we used long-read whole-genome sequencing analysis to identify T-DNA insertion sites in pathogenicity mutants of Colletotrichum higginsianum, a fungus that causes anthracnose on Brassica plants. We identified mutated regions by aligning the sequence reads containing T-DNA to the C. higginsianum reference genome and also detected unexpectedly large chromosomal rearrangements that appear to have been caused by T-DNA transformation. These results demonstrate that long-read whole-genome sequencing is effective for identifying mutated regions in the C. higginsianum mutants.
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This work was supported by JSPS KAKENHI Grant number 20K06055 to TH.
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Tanaka, S., Yoshida, M., Natsume, H. et al. Rapid detection of mutations and chromosomal rearrangements in T-DNA insertion mutants of Colletotrichum higginsianum using long-read whole-genome sequencing. J Gen Plant Pathol 89, 206–210 (2023). https://doi.org/10.1007/s10327-023-01125-5
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DOI: https://doi.org/10.1007/s10327-023-01125-5