Extended Data Fig. 2: RB-TnSeq assay for fungal impacts on bacterial gene fitness.
From: Bacterial–fungal interactions revealed by genome-wide analysis of bacterial mutant fitness
Characterized pooled bacterial mutant libraries were grown in a biofilm either alone or with a fungal partner. After seven days of growth, mutant abundances were compared to the starting library abundances for each condition. Changes in barcode abundances were used to calculate gene fitness values. Genes with fitness values that differed significantly between co-culture and alone conditions (significant interaction fitness) were identified as potentially relevant to fungal interaction.