![Emeritus Laboratory Rudolf Grosschedl Emeritus Laboratory Rudolf Grosschedl](https://cdn.statically.io/img/www.ie-freiburg.mpg.de/1536302/header_image-1615474950.jpg?t=eyJ3aWR0aCI6ODQ4LCJmaWxlX2V4dGVuc2lvbiI6ImpwZyIsIm9ial9pZCI6MTUzNjMwMn0%3D--3303ef7b4e4ab38148530066a82f40e944e3b92b)
Emeritus Laboratory Rudolf Grosschedl
Cellular and Molecular Immunology
Differentiation of hematopoietic stem cells to specialized effector cells involves multiple cell fate choices and a progressive loss of lineage potential. We study how transcription factors change the epigenetic landscape, activate cell type-specific gene expression and ensure the maintenance of lineage identity. We use B lymphopoiesis as a model system and investigate how progenitor cells commit to the B cell lineage. We found that EBF1 acts as a pioneer transcription factor that regulates the transition of naive progenitor chromatin to B-lineage-committed chromatin. In addition, we examine the role of Satb transcription factors in the regulation of higher-order chromatin and differentiation of B cells and embryonic stem cells.
Approach
We use gene editing, genome-wide molecular and cell biological approaches in combination with primary cell transduction and mouse transgenic analysis to gain insight into the regulatory networks of B cell differentiation.
Impact
Our research is aimed at understanding how the correct dosage and function of transcription factors safeguard B lymphoid cells against leukemic transformation.
Selected Publications
Immunity 53(6), 1151-1167.e6