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Scanning Electron Microscopy of Ctenophores: Illustrative Atlas
Scanning electron microscopy (SEM) is a powerful tool for ultrastructural analyses of biological specimens at their surface. With comb jellies being...
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Revealing Ultrastructural Analysis of Starch Using Scanning Electron Microscopy
Starch serves as the main source of energy and carbohydrates in human nutrition. It has a big part in plant foods and offers a lot of desirable...
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Studying the Effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron Microscopy
Glycosaminoglycans, the building blocks of proteoglycans, play a central role in the extracellular matrix and regulate a number of cellular...
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Identification of Ultrastructural Details of the Astrocyte Process System in Nervous Tissue of the Brain Using Correlative Scanning Probe and Transmission Electron Microscopy
AbstractNanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron–astrocyte interactions in...
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Electron Microscopy of Cryptococcus neoformans: Processing Challenges to Avoid Artifacts
This chapter describes methodological details for preparing specimens of Cryptococcus neoformans (although it can be applied to any species of the...
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Assessment of Hardseededness and Its Seasonal Dynamics Through Scanning Electron Microscopy in Green Gram (Vigna radiata) Genotypes
Hardseededness is attributed by impermeable seed coats which mechanically restricts embryo growth and delays germination even under suitable...
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Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities
Plasmodesmata (PD) facilitate the exchange of nutrients and signaling molecules between neighboring plant cells, and they are therefore essential for...
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Revealing Concealed Dimensions of Pollen Fertility by Scanning Electron Microscopy and Palynometabolomic Profiling for Next-Gen Banana Breeding
The cultivated bananas are sexually mysterious owing to the male and female sterility. The sterility factor has long been a hindrance for...
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Some Guiding Principles for a “Simple” Correlative Light Electron Microscopy Experiment
In recent years, Correlative Multimodal Imaging (CMI) has become an “en vogue” technique and a bit of a buzzword. It entails combining information...
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Correlative Light- and Electron Microscopy in Peroxisome Research
Correlative light and electron microscopy (CLEM) combines the advantages of protein localization by fluorescence microscopy with the high resolution...
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Electron Microscopy Techniques for 3D Plant ER Imaging
The endoplasmic reticulum (ER) forms an extensive network in plant cells. In leaf cells and vacuolated root cells it is mainly restricted to the...
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Micro structuring and host tissue patterning of sugarcane grassy shoot phytoplasma through scanning electron microscopy
Sugarcane grassy shoot (SCGS) disease caused by SCGS-phytoplasma is one of the most destructive diseases of sugarcane, which affects all the...
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A semi-automatic method for extracting mitochondrial cristae characteristics from 3D focused ion beam scanning electron microscopy data
Mitochondria are the main suppliers of energy for cells and their bioenergetic function is regulated by mitochondrial dynamics : the constant changes...
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Monitoring dissociation of chimerism through real-time PCR and scanning electron microscopy following in planta transformation of rough lemon (Citrus jambhiri Lush.)
Citrus spp. are recalcitrant to in vitro shoot regeneration and we report an improved in planta protocol for genetic transformation of rough lemon...
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Scanning electron microscopy and morphometric analysis of the hair in dromedaries with SEM-EDX in relation to age
BackgroundHair characterization is critical for determining animal individuality throughout life. This study aimed to assess the morphological...
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Genetically encoded barcodes for correlative volume electron microscopy
While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are...
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Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast, and Plants
Scanning electron microscopy (SEM)Scanning electron microscopy (SEM) can be used to image nuclear pore complex (NPC) surface structure Structures of...
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Characterization of Complexes and Supramolecular Structures by Electron Microscopy
Recent advancements in cryo-electron microscopy (cryo-TEM) have enabled the determination of structures of macromolecular complexes at near-atomic...
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Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines
High-resolution structure determination by electron cryo-microscopy underwent a step change in recent years. This now allows study of challenging...
