https://orcid.org/0000-0002-7523-355X
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Wenbo Lei, Yating Wen, Yewei Yang, Shuangquan Liu, Zhongyu Li, Chlamydia trachomatis T3SS Effector CT622 Induces Proinflammatory Cytokines Through TLR2/TLR4-Mediated MAPK/NF-κB Pathways in THP-1 Cells, The Journal of Infectious Diseases, Volume 229, Issue 6, 15 June 2024, Pages 1637–1647, https://doi.org/10.1093/infdis/jiad597
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Abstract
The pathogenesis of Chlamydia trachomatis is associated with the induction of the host inflammatory response; however, the precise underlying molecular mechanisms remain poorly understood.
CT622, a T3SS effector protein, has an important role in the pathogenesis of C trachomatis; however, whether CT622 can induce a host inflammatory response is not understood. Our findings demonstrate that CT622 induces the expression of interleukins 6 and 8 (IL-6 and IL-8). Mechanistically, these effects involve the activation of the MAPK/NF-κB signaling pathways (mitogen-activated protein kinase/nuclear factor κB).
Interestingly, we demonstrated that the suppression of toll-like receptor 4 using small interfering RNA markedly reduced the phosphorylation of ERK, p38, JNK, and IκBα, concomitant with a significant decrease in IL-6 and IL-8 secretion. Conversely, disruption of toll-like receptor 2 abrogated the CT622-induced upregulation of IL-8 and activation of ERK, whereas IL-6 expression and p38, JNK, and IκBα phosphorylation were unaffected.
Taken together, these results indicate that CT622 contributes to the inflammatory response through the toll-like receptor 2/4–mediated MAPK/NF-κB pathways, which provides insight into the molecular pathology of C trachomatis infection.
