3D Poly (L-lactic acid) fibrous sponge with interconnected porous structure for bone tissue scaffold

Abstract

Large bone defects, often resulting from trauma and disease, present significant clinical challenges. Electrospun fibrous scaffolds closely resembling the morphology and structure of natural ECM are highly interested in bone tissue engineering. However, the traditional electrospun fibrous scaffold has some limitations, including lacking interconnected macropores and behaving as a 2D scaffold. To address these challenges, a sponge-like electrospun poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibrous scaffold has been developed by an innovative and convenient method (i.e., electrospinning, homogenization, progen leaching and shaping). The resulting scaffold exhibited a highly porous structure (overall porosity = 85.9 %) with interconnected, regular macropores, mimicking the natural extracellular matrix. Moreover, the incorporation of bioactive glass (BG) particles improved the hydrophilicity (water contact angle = 79.7°) and biocompatibility and promoted osteoblast cell growth. In-vitro 10-day experiment revealed that the scaffolds led to high cell viability. The increment of the proliferation rates was 195.4 % at day 7 and 281.6 % at day 10. More importantly, Saos-2 cells could grow, proliferate, and infiltrate into the scaffold. Therefore, this 3D PLLA/PCL with BG sponge holds great promise for bone defect repair in tissue engineering applications.

Original language	English
Article number	131688
Journal	International Journal of Biological Macromolecules
Volume	268
Early online date	18 Apr 2024
DOIs	https://doi.org/10.1016/j.ijbiomac.2024.131688
Publication status	Published - 1 May 2024

Keywords

3D monolithic structures
Bone tissue scaffold
Electrospinning
Micro/macroporous
Poly(L-lactic acid)
Polycaprolactone

Research Beacons, Institutes and Platforms

Henry Royce Institute

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10.1016/j.ijbiomac.2024.131688Licence: CC BY

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title = "3D Poly (L-lactic acid) fibrous sponge with interconnected porous structure for bone tissue scaffold",

abstract = "Large bone defects, often resulting from trauma and disease, present significant clinical challenges. Electrospun fibrous scaffolds closely resembling the morphology and structure of natural ECM are highly interested in bone tissue engineering. However, the traditional electrospun fibrous scaffold has some limitations, including lacking interconnected macropores and behaving as a 2D scaffold. To address these challenges, a sponge-like electrospun poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibrous scaffold has been developed by an innovative and convenient method (i.e., electrospinning, homogenization, progen leaching and shaping). The resulting scaffold exhibited a highly porous structure (overall porosity = 85.9 %) with interconnected, regular macropores, mimicking the natural extracellular matrix. Moreover, the incorporation of bioactive glass (BG) particles improved the hydrophilicity (water contact angle = 79.7°) and biocompatibility and promoted osteoblast cell growth. In-vitro 10-day experiment revealed that the scaffolds led to high cell viability. The increment of the proliferation rates was 195.4 % at day 7 and 281.6 % at day 10. More importantly, Saos-2 cells could grow, proliferate, and infiltrate into the scaffold. Therefore, this 3D PLLA/PCL with BG sponge holds great promise for bone defect repair in tissue engineering applications.",

keywords = "3D monolithic structures, Bone tissue scaffold, Electrospinning, Micro/macroporous, Poly(L-lactic acid), Polycaprolactone",

author = "Chen Meng and Xuzhao Liu and Renzhi Li and Samira Malekmohammadi and Yangyang Feng and Jun Song and Hugh Gong and Jiashen Li",

year = "2024",

month = may,

day = "1",

doi = "10.1016/j.ijbiomac.2024.131688",

language = "English",

volume = "268",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier BV",

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TY - JOUR

T1 - 3D Poly (L-lactic acid) fibrous sponge with interconnected porous structure for bone tissue scaffold

AU - Meng, Chen

AU - Liu, Xuzhao

AU - Li, Renzhi

AU - Malekmohammadi, Samira

AU - Feng, Yangyang

AU - Song, Jun

AU - Gong, Hugh

AU - Li, Jiashen

PY - 2024/5/1

Y1 - 2024/5/1

N2 - Large bone defects, often resulting from trauma and disease, present significant clinical challenges. Electrospun fibrous scaffolds closely resembling the morphology and structure of natural ECM are highly interested in bone tissue engineering. However, the traditional electrospun fibrous scaffold has some limitations, including lacking interconnected macropores and behaving as a 2D scaffold. To address these challenges, a sponge-like electrospun poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibrous scaffold has been developed by an innovative and convenient method (i.e., electrospinning, homogenization, progen leaching and shaping). The resulting scaffold exhibited a highly porous structure (overall porosity = 85.9 %) with interconnected, regular macropores, mimicking the natural extracellular matrix. Moreover, the incorporation of bioactive glass (BG) particles improved the hydrophilicity (water contact angle = 79.7°) and biocompatibility and promoted osteoblast cell growth. In-vitro 10-day experiment revealed that the scaffolds led to high cell viability. The increment of the proliferation rates was 195.4 % at day 7 and 281.6 % at day 10. More importantly, Saos-2 cells could grow, proliferate, and infiltrate into the scaffold. Therefore, this 3D PLLA/PCL with BG sponge holds great promise for bone defect repair in tissue engineering applications.

AB - Large bone defects, often resulting from trauma and disease, present significant clinical challenges. Electrospun fibrous scaffolds closely resembling the morphology and structure of natural ECM are highly interested in bone tissue engineering. However, the traditional electrospun fibrous scaffold has some limitations, including lacking interconnected macropores and behaving as a 2D scaffold. To address these challenges, a sponge-like electrospun poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibrous scaffold has been developed by an innovative and convenient method (i.e., electrospinning, homogenization, progen leaching and shaping). The resulting scaffold exhibited a highly porous structure (overall porosity = 85.9 %) with interconnected, regular macropores, mimicking the natural extracellular matrix. Moreover, the incorporation of bioactive glass (BG) particles improved the hydrophilicity (water contact angle = 79.7°) and biocompatibility and promoted osteoblast cell growth. In-vitro 10-day experiment revealed that the scaffolds led to high cell viability. The increment of the proliferation rates was 195.4 % at day 7 and 281.6 % at day 10. More importantly, Saos-2 cells could grow, proliferate, and infiltrate into the scaffold. Therefore, this 3D PLLA/PCL with BG sponge holds great promise for bone defect repair in tissue engineering applications.

KW - 3D monolithic structures

KW - Bone tissue scaffold

KW - Electrospinning

KW - Micro/macroporous

KW - Poly(L-lactic acid)

KW - Polycaprolactone

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DO - 10.1016/j.ijbiomac.2024.131688

M3 - Article

SN - 0141-8130

VL - 268

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

M1 - 131688

ER -

Abstract

Keywords

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