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Mitochondrial DNA Methods and Protocols
This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging,...
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Mitochondrial DNA Analysis
Mitochondrial DNA (mtDNA) is a 16,569 base pair (bp) circular genome that is passed from generation to generation through the maternal line. mtDNA...
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Mitochondrial DNA Isolation from Plants
For most eukaryotes, sequencing and assembly of the mitochondrial DNA (mtDNA) is possible by starting the analysis from total cellular DNA, but the...
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Assessing TFAM Binding to Human Mitochondrial DNA
Mitochondrial transcription factor A (TFAM) is a mitochondrial DNA (mtDNA)-binding protein that plays a crucial dual role in the initiation of...
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Mitochondrial DNA Testing in Assisted Reproduction
In this chapter, the authors explain the role of mitochondrial DNA content to determine oocyte quality and implantation potential to increase the...
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Mitochondrial DNA Enrichment for Sensitive Next-Generation Sequencing
Mitochondrial DNA (mtDNA) encodes components essential for cellular respiration. Low levels of point mutations and deletions accumulate in mtDNA...
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Single Cell Analysis of Mitochondrial DNA Deletions
Mitochondrial DNA (mtDNA) deletions underpin mitochondrial dysfunction in human tissues in aging and disease. The multicopy nature of the...
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Manipulation of Murine Mitochondrial DNA Heteroplasmy with mtZFNs
Mouse models of mitochondrial DNA mutations hold promise in the development and optimization of mitochondrial gene therapy technology and for...
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Regulation with cell size ensures mitochondrial DNA homeostasis during cell growth
To maintain stable DNA concentrations, proliferating cells need to coordinate DNA replication with cell growth. For nuclear DNA, eukaryotic cells...
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Role of Mitochondrial DNA in Yeast Replicative Aging
AbstractDespite the diverse manifestations of aging across different species, some common aging features and underlying mechanisms are shared. In...
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Detection of UV-Induced Deletions in Mitochondrial DNA
Mitochondrial DNA (mtDNA) mutations are found in several human pathologies and are associated with aging. Deletion mutations in mtDNA result in the...
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Using Mitochondrial DNA in Human Identification
Mitochondrial DNA (mtDNA) has been employed for human identification in forensic caseworks since 1996. High copy number and uniparental inheritance...
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Strand-selective base editing of human mitochondrial DNA using mitoBEs
A number of mitochondrial diseases in humans are caused by point mutations that could be corrected by base editors, but delivery of CRISPR guide RNAs...
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Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs
BackgroundDespite their low abundance in sperm, mitochondria have diverse functions in this cell type, including energy production, signalling and...
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Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA
Cellular metabolism relies on the regulation and maintenance of mitochondrial DNA (mtDNA). Hundreds to thousands of copies of mtDNA exist in each...
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Mitochondrial DNA Sequencing and Heteroplasmy Quantification by Next Generation Sequencing
Over the last 10 years, next generation sequencing (NGS) became the gold standard for both diagnosis and discovery of new disease genes responsible...
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TFAM is an autophagy receptor that limits inflammation by binding to cytoplasmic mitochondrial DNA
When cells are stressed, DNA from energy-producing mitochondria can leak out and drive inflammatory immune responses if not cleared. Cells employ a...
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R-Loops and Mitochondrial DNA Metabolism
R-loops forming inadvertently during transcription can threaten genome stability, but R-loops are also formed intentionally, as a means of regulating...
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Mitochondrial DNA release via the mitochondrial permeability transition pore activates the cGAS-STING pathway, exacerbating inflammation in acute Kawasaki disease
BackgroundKawasaki disease (KD) is an immune vasculitis of unknown origin, characterized by transient inflammation. The activation of the cGAS-STING...
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Horizontal transfer of tRNA genes to mitochondrial plasmids facilitates gene loss from fungal mitochondrial DNA
Fungal and plant mitochondria are known to exchange DNA with retroviral plasmids. Transfer of plasmid DNA to the organellar genome is best known and...
