PTPRE, CPTAC-1569 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PTPRE	N/A

Sequence Length (AA)	Molecular Weight (Da)
700	80642

Protein Name
Receptor-type tyrosine-protein phosphatase epsilon

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEPLCPLLLV	GFSLPLARAL	RGNETTADSN	ETTTTSGPPD	PGASQPLLAW
60	70	80	90	100
LLLPLLLLLL	VLLLAAYFFR	FRKQRKAVVS	TSDKKMPNGI	LEEQEQQRVM
110	120	130	140	150
LLSRSPSGPK	KYFPIPVEHL	EEEIRIRSAD	DCKQFREEFN	SLPSGHIQGT
160	170	180	190	200
FELANKEENR	EKNRYPNILP	NDHSRVILSQ	LDGIPCSDYI	NASYIDGYKE
210	220	230	240	250
KNKFIAAQGP	KQETVNDFWR	MVWEQKSATI	VMLTNLKERK	EEKCHQYWPD
260	270	280	290	300
QGCWTYGNIR	VCVEDCVVLV	DYTIRKFCIQ	PQLPDGCKAP	RLVSQLHFTS
310	320	330	340	350
WPDFGVPFTP	IGMLKFLKKV	KTLNPVHAGP	IVVHCSAGVG	RTGTFIVIDA
360	370	380	390	400
MMAMMHAEQK	VDVFEFVSRI	RNQRPQMVQT	DMQYTFIYQA	LLEYYLYGDT
410	420	430	440	450
ELDVSSLEKH	LQTMHGTTTH	FDKIGLEEEF	RKLTNVRIMK	ENMRTGNLPA
460	470	480	490	500
NMKKARVIQI	IPYDFNRVIL	SMKRGQEYTD	YINASFIDGY	RQKDYFIATQ
510	520	530	540	550
GPLAHTVEDF	WRMIWEWKSH	TIVMLTEVQE	REQDKCYQYW	PTEGSVTHGE
560	570	580	590	600
ITIEIKNDTL	SEAISIRDFL	VTLNQPQARQ	EEQVRVVRQF	HFHGWPEIGI
610	620	630	640	650
PAEGKGMIDL	IAAVQKQQQQ	TGNHPITVHC	SAGAGRTGTF	IALSNILERV
660	670	680	690	700
KAEGLLDVFQ	AVKSLRLQRP	HMVQTLEQYE	FCYKVVQDFI	DIFSDYANFK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1569 Collapse assay details

Data source: Panorama

Official Gene Symbol: PTPRE
Peptide Sequence: TGTFIALSNILER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 637
Peptide End: 649

CPTAC ID: CPTAC-1569
Peptide Molecular Mass: 1,433.7878
Species: Homo sapiens (Human)
Assay Type: Direct MRM or SRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2

CPTAC-1538: PIK3R2. ALGATFGPLLLR	CPTAC-1539: PIK3R1. FSAASSDNTENLIK	CPTAC-1540: PDPK1. QLLLTEGPHLYYVDPVNK
CPTAC-1541: MAPK1. VADPDHDHTGFLTEYVATR	CPTAC-1542: MAPK3. IADPEHDHTGFLTEYVATR	CPTAC-1543: MAP2K2. ISELGAGNGGVVTK
CPTAC-1544: MAP2K1. ISELGAGNGGVVFK	CPTAC-1545: MAP2K1. LIHLEIKPAIR	CPTAC-1546: RAF1. TISNGFGFK
CPTAC-1547: ARAF. TQHC[+57.0]DPEHFPFPAPANAPLQR	CPTAC-1548: RASA1. LLAITELLQQK	CPTAC-1549: NRAS. SFADINLYR
CPTAC-1550: KRAS. SFEDIHHYR	CPTAC-1551: HRAS. LVVVGAGGVGK	CPTAC-1552: HRAS. SFEDIHQYR
CPTAC-1553: SOS2. LPGYSSAEYR	CPTAC-1554: SOS1. LPSADVYR	CPTAC-1555: SOS1. EYIQPVQLR
CPTAC-1556: PTPN11. SNPGDFTLSVR	CPTAC-1557: GAB1. LTGDPDVLEYYK	CPTAC-1558: GRB2. ATADDELSFK
CPTAC-1559: SHC1. EAISLVC[+57.0]EAVPGAK	CPTAC-1560: EGFR. LTQLGTFEDHFLSLQR	CPTAC-1561: ADAM17. NIYLNSGLTSTK
CPTAC-1562: SHOC2. LDSLTTLYLR	CPTAC-1563: SPRED1. VPHQEENGC[+57.0]ADFFIR	CPTAC-1564: DUSP6. DSTNLDVLEEFGIK
CPTAC-1565: DUSP4. GSVSLEQILPAEEEVR	CPTAC-1566: ERRFI1. NSPSLFPC[+57.0]APLC[+57.0]ER	CPTAC-1567: CBL. GTEPIVVDPFDPR
CPTAC-1568: PEBP1. VLTPTQVK	CPTAC-1569: PTPRE. TGTFIALSNILER	CPTAC-1570: SPRY4. LQHPLTILPIDQVK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	16.2	4	4.6	27.3	6.4	8.2	31.7	7.5	9.4	15	15	15
y8 (1+)	9.6	6.1	3.1	26.7	8.2	7.9	28.4	10.2	8.5	15	15	15
y7 (1+)	13.4	7.1	4	25.3	8.4	8.4	28.6	11	9.3	15	15	15
y6 (1+)	10.8	4.4	4.9	23.6	6	7.7	26	7.4	9.1	15	15	15
sum	6.4	4.7	3	24.6	7	7.7	25.4	8.4	8.3	15	15	15

PTPRE

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1569 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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