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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIK3R2 N/A
Sequence Length (AA) Molecular Weight (Da)
728 81545
Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGGERC
60 70 80 90 100
PQSVGWMPGL NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP
110 120 130 140 150
RDGAPEPGLT LPDLPEQFSP PDVAPPLLVK LVEAIERTGL DSESHYRPEL
160 170 180 190 200
PAPRTDWSLS DVDQWDTAAL ADGIKSFLLA LPAPLVTPEA SAEARRALRE
210 220 230 240 250
AAGPVGPALE PPTLPLHRAL TLRFLLQHLG RVASRAPALG PAVRALGATF
260 270 280 290 300
GPLLLRAPPP PSSPPPGGAP DGSEPSPDFP ALLVEKLLQE HLEEQEVAPP
310 320 330 340 350
ALPPKPPKAK PASTVLANGG SPPSLQDAEW YWGDISREEV NEKLRDTPDG
360 370 380 390 400
TFLVRDASSK IQGEYTLTLR KGGNNKLIKV FHRDGHYGFS EPLTFCSVVD
410 420 430 440 450
LINHYRHESL AQYNAKLDTR LLYPVSKYQQ DQIVKEDSVE AVGAQLKVYH
460 470 480 490 500
QQYQDKSREY DQLYEEYTRT SQELQMKRTA IEAFNETIKI FEEQGQTQEK
510 520 530 540 550
CSKEYLERFR REGNEKEMQR ILLNSERLKS RIAEIHESRT KLEQQLRAQA
560 570 580 590 600
SDNREIDKRM NSLKPDLMQL RKIRDQYLVW LTQKGARQKK INEWLGIKNE
610 620 630 640 650
TEDQYALMED EDDLPHHEER TWYVGKINRT QAEEMLSGKR DGTFLIRESS
660 670 680 690 700
QRGCYACSVV VDGDTKHCVI YRTATGFGFA EPYNLYGSLK ELVLHYQHAS
710 720 728
LVQHNDALTV TLAHPVRAPG PGPPPAAR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1538 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3R2
Peptide Sequence
ALGATFGPLLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
245
Peptide End
256
CPTAC ID
CPTAC-1538
Peptide Molecular Mass
1,227.7339
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 12.5 4.2 3.7 19.7 4.9 5.4 23.3 6.5 6.5 15 15 15
y6 (1+) 5.7 3.9 4.5 14.7 4.2 6.9 15.8 5.7 8.2 15 15 15
y5 (1+) 15.5 4.2 4.7 19.7 5.2 5.9 25.1 6.7 7.5 15 15 15
sum 5 3.3 2.9 15.2 4 5.6 16 5.2 6.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2802 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3R2
Peptide Modified Sequence
GC[+57.0]YAC[+57.0]SVVVDGDTK
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
654, 657
Peptide - Site of Modification
2, 5
Peptide Start
653
Peptide End
666
CPTAC ID
CPTAC-2802
Peptide Molecular Mass
1,529.6490
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.5 2.1 7.7 5.9 4.4 8 8.1 4.9 11.1 15 15 15
y5 (1+) 6.6 3.7 6.8 7.3 4.8 9.1 9.8 6.1 11.4 15 15 15
y4 (1+) 4 3 5.9 4.1 3.8 7.3 5.7 4.8 9.4 15 15 15
sum 3.6 2.3 6.7 4.7 4 8 5.9 4.6 10.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2801 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3R2
Peptide Sequence
TATGFGFAEPYNLYGSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
673
Peptide End
690
CPTAC ID
CPTAC-2801
Peptide Molecular Mass
1,934.9414
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectSRM2


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (1+) 4.9 4.7 9.1 8.7 10.3 9.9 10 11.3 13.4 15 15 15
y11 (1+) 4.8 4.3 8.7 7.5 9.3 9 8.9 10.2 12.5 15 15 15
y10 (1+) 5.8 4.3 8.8 8.3 8.1 10.5 10.1 9.2 13.7 15 15 15
sum 4.4 4.2 8.7 7.9 9.2 9.6 9 10.1 13 15 15 15


Additional Resources and Comments