Milap Thaker - Biology Powerpoint: Harvard University - DNA Damage Checkpoints

The document discusses two mechanisms by which DNA damage checkpoints inhibit mitotic exit in yeast cells. First, the Rad53 checkpoint kinase prevents mitotic exit by inhibiting the Mitotic Exit Network (MEN). Second, the FEAR pathway promotes limited release of the phosphatase Cdc14 from the nucleolus early in anaphase. The study finds that Rad53 acts through the Dun1 kinase to regulate the MEN more directly, while FEAR provides an alternate mechanism for temporary Cdc14 release and a delay in full mitotic exit. Experiments visualize budding, Cdc14 localization, and DNA content in various yeast strains to illustrate the two inhibitory mechanisms.Read less