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| Status |
Public on May 14, 2023 |
| Title |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia [scMultiome] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study characterized the effect of WEE1 kinase inhibition using AZD1775 treatment on single-cell accessible chromatin and gene expression profile using the 10x Genomics multiome protocol (ATAC + Gene Expression Assay) in the acute lymphoblastic leukemia cell line RS4;11 that represents the KMT2A-rearranged subtype.
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| Overall design |
scMultiome-seq samples were generated from cells treated for 10 h with AZD1775(Wee1i) or vehicle (DMSO) using the 10x Genomics Chromium platform and protocols generating scRNA-seq and scATAC-seq from the same cells.
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| Contributor(s) |
Lahnalampi M, Heinäniemi M, Malyukova A, Sangfelt O |
| Citation(s) |
38822412 |
| |
| Submission date |
Dec 05, 2022 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Merja Heinäniemi |
| E-mail(s) |
merja.heinaniemi@uef.fi
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| Organization name |
University of Eastern Finland
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| Department |
School of Medicine, Institute of Biomedicine
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| Lab |
Systems Genomics - Heinäniemi lab
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| Street address |
Yliopistonranta 1E
|
| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE220114 |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia |
|
| Relations |
| BioProject |
PRJNA908817 |
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