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Status |
Public on May 14, 2023 |
Title |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia [scATAC-seq] |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
This study characterized the effect of WEE1 kinase inhibition using AZD1775 treatment on single-cell accessible chromatin and gene expression profile in the acute lymphoblastic leukemia cell line RS4;11 that represents the KMT2A-rearranged subtype.
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Overall design |
scATAC-seq samples were generated from 24 h after AZD1775 (Wee1i) using the 10x Genomics Chromium platform and protocols. DMSO treated cells were used as controls. Parallel cell cultures were processed for scRNA-seq.
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Contributor(s) |
Lahnalampi M, Heinäniemi M, Malyukova A, Sangfelt O |
Citation(s) |
38822412 |
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Submission date |
Nov 25, 2022 |
Last update date |
Jun 26, 2024 |
Contact name |
Merja Heinäniemi |
E-mail(s) |
merja.heinaniemi@uef.fi
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Organization name |
University of Eastern Finland
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Department |
School of Medicine, Institute of Biomedicine
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Lab |
Systems Genomics - Heinäniemi lab
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Street address |
Yliopistonranta 1E
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City |
Kuopio |
ZIP/Postal code |
70211 |
Country |
Finland |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE220114 |
Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia |
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Relations |
BioProject |
PRJNA905534 |