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Series GSE151827 Query DataSets for GSE151827
Status Public on Sep 22, 2020
Title Non-CG methylation and multiple epigenetic layers associate child abuse with immune and small GTPase dysregulation
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Early-life adversity (ELA) is a major predictor of psychopathology, and is thought to increase lifetime risk by epigenetically regulating the genome. Here, focusing on the lateral amygdala, a major brain site for emotional homeostasis, we described molecular cross-talk among multiple epigenetic mechanisms, including 6 histone marks, DNA methylation and the transcriptome, in subjects with a history of ELA and controls. We first uncovered, in the healthy brain, previously unknown interactions among epigenetic layers, in particular related to non-CG methylation in the CAC context. We then showed that ELA associates with methylomic changes that are as frequent in the CAC as in the canonical CG context, while these two forms of plasticity occur in sharply distinct genomic regions, features, and chromatin states. Combining these multiple data indicated that immune-related and small GTPase signaling pathways are most consistently impaired in the amygdala of ELA individuals. Overall, this work provides new insights into epigenetic brain regulation as a function of early-life experience.
 
Overall design Examination of gene expression, DNA methylation and 6 histone marks in human brain tissue samples from the lateral amygdala, and comparison to gene expression data generated in another brain region, the anterior cingulate cortex (data first reported in Lutz, Tanti et al, The American Journal Of Psychiatry 2017, 174:12)
 
Contributor(s) Lutz P, Chay M, Turecki G, Théroux J
Citation(s) 33602921, 34385599, 37069164
Submission date Jun 04, 2020
Last update date Apr 26, 2023
Contact name Pierre-Eric Lutz
E-mail(s) pierreeric.lutz@gmail.com
Phone +33388456729
Organization name Institut des Neurosciences Cellulaires et Intégratives, INCI CNRS UPR3212
Lab Pain & psychopathology
Street address 8 allée du général Rouvillois
City Strasbourg
ZIP/Postal code 67000
Country France
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (203)
GSM4591775 Lateral amygdala, S1, WGBS
GSM4591776 Lateral amygdala, S2, WGBS
GSM4591777 Lateral amygdala, S3, WGBS
Relations
BioProject PRJNA637378
SRA SRP266187

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Supplementary file Size Download File type/resource
GSE151827_ACC_RNASeq.rawcounts.tsv.gz 2.4 Mb (ftp)(http) TSV
GSE151827_LatAmy_Brain_RNASeq.raw.counts.txt.gz 2.1 Mb (ftp)(http) TXT
GSE151827_RAW.tar 251.0 Gb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record
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