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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 20, 2019 |
| Title |
Transcriptional characterization of mPFCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Medial prefrontal cortices (mPFCs) were dissected from the brain of control or antibiotic-treated 10 week old male mice, before or after fear conditioning and extinction learning for transcriptional analysis.
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| Overall design |
Mouse mPFCs were dissected by referring to the Allen Brain Atlas. Coordinates relative to bregma are: 1.3 to 2.8 mm anterior, -1 to 1 mm lateral, and 0 to 1 mm ventral. RNA was extracted using Trizol (Invitrogen) and chloroform and further purified using the RNAeasy mini spin columns (Qiagen).
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| Contributor(s) |
Chu C, Artis D |
| Citation(s) |
31645720 |
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| Submission date |
Jul 24, 2019 |
| Last update date |
Feb 16, 2021 |
| Contact name |
Gregory Putzel |
| Organization name |
NYU Langone Health
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| Department |
Microbiology
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| Lab |
Pironti
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| Street address |
430 East 29th Street
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| GSM3971994 |
Pre-fear-extinction, ABX-treated sample 1 |
| GSM3971995 |
Pre-fear-extinction, ABX-treated sample 2 |
| GSM3971996 |
Pre-fear-extinction, ABX-treated sample 3 |
| GSM3971997 |
Pre-fear-extinction, ABX-treated sample 4 |
| GSM3971998 |
Pre-fear-extinction, non-ABX-treated sample 1 |
| GSM3971999 |
Pre-fear-extinction, non-ABX-treated sample 3 |
| GSM3972000 |
Pre-fear-extinction, non-ABX-treated sample 4 |
| GSM3972001 |
Post-fear-extinction, ABX-treated sample 1 |
| GSM3972002 |
Post-fear-extinction, ABX-treated sample 2 |
| GSM3972003 |
Post-fear-extinction, ABX-treated sample 3 |
| GSM3972004 |
Post-fear-extinction, ABX-treated sample 4 |
| GSM3972005 |
Post-fear-extinction, non-ABX-treated sample 1 |
| GSM3972006 |
Post-fear-extinction, non-ABX-treated sample 2 |
| GSM3972007 |
Post-fear-extinction, non-ABX-treated sample 3 |
| GSM3972008 |
Post-fear-extinction, non-ABX-treated sample 4 |
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| Relations |
| BioProject |
PRJNA556460 |
| SRA |
SRP216264 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134808_gene_counts.tsv.gz |
611.3 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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