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| Status |
Public on Oct 22, 2020 |
| Title |
The order and stochasticity in the Drosophila genome folding revealed by single-nucleus Hi-C |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
We generated and analyzed single-cell Hi-C libraries from cultrured Drosophila cells. The data obtained allowed us to explore cell-to-cell variability in TAD profiles and inter-TAD interactions showing that strong TAD boundaries are determined by high levels of active chromatin. DPD polymer simulations were utilized to reconstruct 3D structures of haploid X chromosomes. These structures showed marked cell-to-cell variabilty in the overall shape of the chromosome territory and relatively conserved chromatin chain path inside TADs.
DPD polymer simulations were utilized to reconstruct 3D structures of haploid X chromosomes.
These structures showed marked cell-to-cell variabilty in the overall shape of the chromosome territory and relatively conserved chromatin chain path inside TADs.
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| Overall design |
We generated bulk and single-cell Hi-C libraries for Drosophila melanogaster for 88 ML-DmBG3-c2 cells
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| Contributor(s) |
Galitsyna A, Zakharova V |
| Citation(s) |
33397980 |
| |
| Submission date |
May 28, 2019 |
| Last update date |
Jul 28, 2023 |
| Contact name |
Aleksandra Galitsyna |
| E-mail(s) |
agalitzina@gmail.com
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
IMES
|
| Lab |
Leonid Mirny lab
|
| Street address |
77 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (89)
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| Relations |
| BioProject |
PRJNA545053 |
| SRA |
SRP199618 |
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