Methods Protoc., Volume 7, Issue 3 (June 2024) – 14 articles
Cover Story (view full-size image):
Timely and accurate characterization of microbial threats is essential for infection control and food safety. Traditional methods for microbial strain typing, which rely on DNA- and sera-serotyping techniques, are often slow and costly, limiting their applicability in rapid-response scenarios. The IR Biotyper®, utilizing Fourier-transform infrared (FTIR) spectroscopy, offers a novel approach, providing specific spectra for fast strain typing within 3 hours. This article guides researchers and technicians in using FTIR spectroscopy for microbial strain typing, covering sample preparation, data acquisition, and analysis. We highlight FTIR spectroscopy's potential for real-time pathogen monitoring and propose its integration with whole-genome sequencing for enhanced microbial surveillance. View this paper
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