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Happy National Intern Day to our amazing interns at identifeye HEALTH! The identifeye HEALTH internship program provides students and early career…
Happy National Intern Day to our amazing interns at identifeye HEALTH! The identifeye HEALTH internship program provides students and early career…
Liked by Vasiliki (Vicky) Demas
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11.6% of the US population has diabetes, according to the CDC, and about 1/3 of people with diabetes will develop diabetic retinopathy, the leading…
11.6% of the US population has diabetes, according to the CDC, and about 1/3 of people with diabetes will develop diabetic retinopathy, the leading…
Shared by Vasiliki (Vicky) Demas
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During this year’s Clinical Trials at the Summit meeting, Dr. Jay Pepose, the chief medical advisor for Ocuphire Pharma, highlighted the progress of…
During this year’s Clinical Trials at the Summit meeting, Dr. Jay Pepose, the chief medical advisor for Ocuphire Pharma, highlighted the progress of…
Shared by Vasiliki (Vicky) Demas
Experience & Education
Publications
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A comprehensive characterization of the cell-free transcriptome reveals tissue-and subtype-specific biomarkers for cancer detection (vol 12, 2357, 2021)
Nature Communications
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A comprehensive characterization of the cell-free transcriptome reveals tissue-and subtype-specific biomarkers for cancer detection
Nature communications
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Nuclear Magnetic Resonance Nanotechnology: Applications in Clinical Diagnostics and Monitoring
Encyclopedia of Analytical Chemistry
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High Resolution NMR in Inhomogeneous Fields
Book Chapter in Single-Sided NMR Book by Springer
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Magnetic Resonance for In Vitro Medical Diagnostics: Superparamagnetic Nanoparticle-Based Magnetic Relaxation Switches
New Journal of Physics 13 025005
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Distortion-free magnetic resonance imaging in the zero-field limit.
Journal of Magnetic Resonance 2009 Oct 18;200(2):285-90.
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Least Squares Magnetic-Field Optimization for Portable Nuclear Magnetic Resonance Magnet Design
IEEE Transactions on Magnetics, 44 (2008) 4582-4590
Other authors -
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Experimental Results to Assist in Validation of Casting Process Simulations in a Variable-Gravity Environment
Transactions of the American Foundry Society V 111 Paper No 03-091 P 29-39, 2003 (11 p)
Producing objects directly from the liquid state of materials is one of the important manufacturing processes and one deserving significant attention. It is an unfortunate fact that few scientific efforts and outcomes have been directed toward the optimization of such an important process for manufacturing as casting. Progress has been made however, particularly with the advance of computer modeling adding a new dimension to the ability to predict and control its variables affecting casting…
Producing objects directly from the liquid state of materials is one of the important manufacturing processes and one deserving significant attention. It is an unfortunate fact that few scientific efforts and outcomes have been directed toward the optimization of such an important process for manufacturing as casting. Progress has been made however, particularly with the advance of computer modeling adding a new dimension to the ability to predict and control its variables affecting casting processes and the resulting quality. The goal of this effort has been to use small, but important, segments of applied science directed toward providing insights and data, which may assist the improvement of computer codes and further contribute to the casting industry.
Other authors -
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Experimental Design of a Microgravity Solidification Facility to Assist with Casting Simulation Verification
Transactions of the American Foundry Society V 109 Paper No 01-022, P 1-7, 2001 (7 p)
Computer simulation of the casting process is a cost-effective way of predicting foundry process design outcomes. An objective of this project is to validate the capability of commercially available simulation software packages to predict the phenomena that occur during casting with or without considering gravitational forces. The validity of the results can be confirmed in experiments with appropriate analog systems, performed in variable gravity environments. The gravity profile in NASA's…
Computer simulation of the casting process is a cost-effective way of predicting foundry process design outcomes. An objective of this project is to validate the capability of commercially available simulation software packages to predict the phenomena that occur during casting with or without considering gravitational forces. The validity of the results can be confirmed in experiments with appropriate analog systems, performed in variable gravity environments. The gravity profile in NASA's KC-135 aircraft, and the experimental apparatus that is used to observe the solidification and fluid flow characteristics of a metal analog in KC-135 environment are described in this paper.
Other authors -
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Validation of Casting Process Simulations Using Analog Materials in a Microgravity Environment
Transactions of the American Foundry Society V 109 Paper No 01-173 P 1-14, 2001 (14 p)
Producing objects directly from the liquid state of materials is one of the important manufacturing processes and one deserving significant attention. It is an unfortunate fact that little of the scientific efforts and fruits have been directed toward the optimization of such an important process for manufacturing as casting. Progress has been made however, particularly with the advance of computer modeling adding a new dimension to the ability to predict and control its variables affecting…
Producing objects directly from the liquid state of materials is one of the important manufacturing processes and one deserving significant attention. It is an unfortunate fact that little of the scientific efforts and fruits have been directed toward the optimization of such an important process for manufacturing as casting. Progress has been made however, particularly with the advance of computer modeling adding a new dimension to the ability to predict and control its variables affecting casting processes and the resulting quality. The goal of this effort has been to use small but important segments of applied science directed toward providing insights and data, which may assist the improvement of computer codes and further contribute to the casting industry.
Other authors
Patents
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Containers for agitation of liquid samples and methods of use thereof
Issued US 11666872
The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.
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Device, system, and formulation for oral delivery of functionalized particles
Issued US 11660436
A device includes a capsule sized to pass through a lumen of a gastrointestinal tract; an enteric coating surround at least a portion of the capsule and configured to protect the capsule form stomach acid while allowing degradation of the capsule in the small intestine of the gastrointestinal tract; a plurality of functionalized particles disposed within the capsule, a plurality of tissue penetrating members configured to puncture a wall of the lumen of the intestinal tract; and an actuator…
A device includes a capsule sized to pass through a lumen of a gastrointestinal tract; an enteric coating surround at least a portion of the capsule and configured to protect the capsule form stomach acid while allowing degradation of the capsule in the small intestine of the gastrointestinal tract; a plurality of functionalized particles disposed within the capsule, a plurality of tissue penetrating members configured to puncture a wall of the lumen of the intestinal tract; and an actuator having a first configuration and a second configuration. The actuator is configured to retain the plurality of functionalized particles within the capsule in the first configuration. The actuator is further configured to advance the plurality of functionalized particles from the capsule into a wall of the lumen of the gastrointestinal tract via the plurality of tissue penetrating members by the actuator transitioning from the first configuration to the second configuration.
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In-vivo magnetic detection of magnetic nanoparticles using microneedles
Issued 11039789
Systems and methods are described that enable sensing of magnetic fields within skin tissue. Specifically, a system includes one or more microneedles that include a high magnetic permeability material. The system also includes a magnetic sensor communicatively coupled to the microneedle and configured to detect a magnetic field proximate to the microneedle. The system also includes a controller configured to receive information indicative of a magnetic field proximate to a portion of the…
Systems and methods are described that enable sensing of magnetic fields within skin tissue. Specifically, a system includes one or more microneedles that include a high magnetic permeability material. The system also includes a magnetic sensor communicatively coupled to the microneedle and configured to detect a magnetic field proximate to the microneedle. The system also includes a controller configured to receive information indicative of a magnetic field proximate to a portion of the microneedle. The controller is further configured to determine a presence of at least one magnetic nanoparticle proximate to the portion of the microneedle based on the received information. Alternatively, other embodiments include a microneedle that includes a nanodiamond material configured to detect a local magnetic field. Such embodiments also include a light source configured to cause the nanodiamond material to emit characteristic emission light that may indicate at least a magnitude of the magnetic field.
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Containers for agitation of liquid samples and methods of use thereof
Issued 10486118
The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.
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Magnetic Switching
Issued US 10349870
Methods of exerting magnetic forces to collect and manipulate magnetic particles disposed in a portion of subsurface vasculature using a wearable device are provided. The wearable device is configured to change the exerted magnetic force over time. For example, the exerted magnetic force could be sufficient to collect the magnetic particles during a first period of time and low enough to release the magnetic particles during a second period of time. The exerted magnetic force could be changed…
Methods of exerting magnetic forces to collect and manipulate magnetic particles disposed in a portion of subsurface vasculature using a wearable device are provided. The wearable device is configured to change the exerted magnetic force over time. For example, the exerted magnetic force could be sufficient to collect the magnetic particles during a first period of time and low enough to release the magnetic particles during a second period of time. The exerted magnetic force could be changed over time to vary some effect on the magnetic particles, for example to control a rate of release of collected magnetic particles. In some embodiments, the magnetic particles are configured to bind to an analyte of interest. The collection and manipulation of the magnetic particles can enable detection of one or more properties of the analyte, modification of the analyte, and/or extraction of the analyte bound to the magnetic particles.
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Segmented Magnets
Issued US 9999380
A variety of wearable magnetic assemblies are provided that are configured to produce magnetic fields having high field magnitudes and/or high field gradients. Such magnetic assemblies include a plurality of magnetic segments arranged in a linear array. Individual magnetic segments of the magnetic array can each include multiple magnetic elements. An individual magnetic segment can include elements that have similar shape, size, composition, and relative location to elements of neighboring…
A variety of wearable magnetic assemblies are provided that are configured to produce magnetic fields having high field magnitudes and/or high field gradients. Such magnetic assemblies include a plurality of magnetic segments arranged in a linear array. Individual magnetic segments of the magnetic array can each include multiple magnetic elements. An individual magnetic segment can include elements that have similar shape, size, composition, and relative location to elements of neighboring magnetic segments while having magnetic moments that are antiparallel to the magnetic moments of corresponding elements of the neighboring magnetic segments. These wearable magnetic assemblies are configured to exert forces on magnetic particles disposed in a portion of subsurface vasculature to attract, slow, speed, separate, or otherwise influence the magnetic particles in various applications. The magnetic particles can be configured to bind to an analyte of interest.
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Analyte detection system and method for intradermal implantation of biocompatible optode nanosensors
Issued US 9974471
A device and system for measuring and/or monitoring an analyte present on the skin is provided. The system includes a skin-mountable device that may be attached to an external skin surface and a reader device. The skin-mountable device includes a substrate, a plurality of micro-needles, and nanosensors. The micro-needles are attached to the substrate such that attachment of the substrate to an external skin surface causes to the micro-needles to penetrate into the epidermis, intradermis, or…
A device and system for measuring and/or monitoring an analyte present on the skin is provided. The system includes a skin-mountable device that may be attached to an external skin surface and a reader device. The skin-mountable device includes a substrate, a plurality of micro-needles, and nanosensors. The micro-needles are attached to the substrate such that attachment of the substrate to an external skin surface causes to the micro-needles to penetrate into the epidermis, intradermis, or dermis. The nanosensors include a detectable label and are configured to interact with a target analyte present in the interstitial fluid in the epidermis, intradermis, or dermis. The reader device is configured to detect the analyte in interstitial fluid via interaction with the skin-mountable device.
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Nanodiamond Counting
Issued US 9897603
Methods and systems for detecting the locations of individual instances of an analyte (e.g., individual cells, individual molecules) in an environment are provided. The environment includes functionalized fluorophores that are configured to selective interact with (e.g., bind with) the analyte and that have a fluorescent property that can be modulated (e.g., a fluorescence intensity that can be affected by the presence of a magnetic field). Detecting the location of individual instances of the…
Methods and systems for detecting the locations of individual instances of an analyte (e.g., individual cells, individual molecules) in an environment are provided. The environment includes functionalized fluorophores that are configured to selective interact with (e.g., bind with) the analyte and that have a fluorescent property that can be modulated (e.g., a fluorescence intensity that can be affected by the presence of a magnetic field). Detecting the location of individual instances of the analyte includes illuminating the environment and detecting signals emitted from the fluorophores in response to the illumination during first and second periods of time. Detecting the location of individual instances of the analyte further includes modulating the modulatable fluorescent property of the fluorophores during the second period of time and determining which individual fluorophores in the environment are bound to the analyte based on the signals detected during the first and second periods of time.
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Composite particles, methods, and in vivo diagnostic system
Issued US 9861710
A system is provided which includes the composite nanoparticles configured to bind with a target analyte, the composite nanoparticles including a polymer matrix; nanoparticles at least one type; reporter labels at least one type; and targeting entities at least one type, wherein the nanoparticles at least one type, the reporter labels at least one type and the targeting entities at least one type are encapsulated in the polymer matrix; a body-mountable device mounted on an external surface of a…
A system is provided which includes the composite nanoparticles configured to bind with a target analyte, the composite nanoparticles including a polymer matrix; nanoparticles at least one type; reporter labels at least one type; and targeting entities at least one type, wherein the nanoparticles at least one type, the reporter labels at least one type and the targeting entities at least one type are encapsulated in the polymer matrix; a body-mountable device mounted on an external surface of a living body and configured to detect a target analyte binding response signal transmitted through the external surface, wherein the target analyte binding response signal is related to binding of the composite nanoparticles with one or more target analytes; and a processor configured to non-invasively detect the one or more target analytes based on the target analyte response signal. Composite nanoparticles and methods for use and for making are also provided.
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Methods for magnetic particle capture and separation
Issued US 9788763
Methods of exerting magnetic forces to separate magnetic particles disposed in a portion of subsurface vasculature using a wearable device are provided. The magnetic forces can act to attract, slow, speed, or otherwise influence the magnetic particles in various applications. In some examples, different magnetic forces are exerted on respective sets of magnetic particles to separate the respective sets of magnetic particles. In some examples, similar magnetic forces are exerted on sets of…
Methods of exerting magnetic forces to separate magnetic particles disposed in a portion of subsurface vasculature using a wearable device are provided. The magnetic forces can act to attract, slow, speed, or otherwise influence the magnetic particles in various applications. In some examples, different magnetic forces are exerted on respective sets of magnetic particles to separate the respective sets of magnetic particles. In some examples, similar magnetic forces are exerted on sets of magnetic particles, and separation of the sets of magnetic particles is related to properties of the sets of magnetic particles and/or of the environment of the sets of magnetic particles. In some embodiments, the magnetic particles are configured to bind to an analyte of interest. The separation of the magnetic particles can enable detection of one or more properties of the analyte, modification of the analyte, and/or extraction of the analyte bound to the magnetic particles.
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Particle concentration and separation using magnets
Issued US 9770600
A variety of wearable magnetic assemblies are provided that are configured to produce magnetic fields having high field magnitudes and/or high field gradients. These wearable magnetic assemblies are configured to exert forces on magnetic particles disposed in a portion of subsurface vasculature (e.g., a portion of the ulnar artery near the wrist) proximate to the magnetic assemblies. These magnetic assemblies include a plurality of dipolar permanent magnets. The forces can act to attract, slow,…
A variety of wearable magnetic assemblies are provided that are configured to produce magnetic fields having high field magnitudes and/or high field gradients. These wearable magnetic assemblies are configured to exert forces on magnetic particles disposed in a portion of subsurface vasculature (e.g., a portion of the ulnar artery near the wrist) proximate to the magnetic assemblies. These magnetic assemblies include a plurality of dipolar permanent magnets. The forces can act to attract, slow, speed, separate, or otherwise influence the magnetic particles in various applications. In some embodiments, the magnetic particles are configured to bind to an analyte of interest. The collection, separation, and/or concentration of the magnetic particles can enable detection of one or more properties of the analyte, modification of the analyte, and/or extraction of the analyte bound to the magnetic particles.
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Nanodiamond counting
Issued US 9759719
Methods and systems for detecting the locations of individual instances of an analyte (e.g., individual cells, individual molecules) in an environment are provided. The environment includes functionalized fluorophores that are configured to selective interact with (e.g., bind with) the analyte and that have a fluorescent property that can be modulated (e.g., a fluorescence intensity that can be affected by the presence of a magnetic field). Detecting the location of individual instances of the…
Methods and systems for detecting the locations of individual instances of an analyte (e.g., individual cells, individual molecules) in an environment are provided. The environment includes functionalized fluorophores that are configured to selective interact with (e.g., bind with) the analyte and that have a fluorescent property that can be modulated (e.g., a fluorescence intensity that can be affected by the presence of a magnetic field). Detecting the location of individual instances of the analyte includes illuminating the environment and detecting signals emitted from the fluorophores in response to the illumination during first and second periods of time. Detecting the location of individual instances of the analyte further includes modulating the modulatable fluorescent property of the fluorophores during the second period of time and determining which individual fluorophores in the environment are bound to the analyte based on the signals detected during the first and second periods of time.
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NMR systems and methods for the rapid detection of analytes
Issued US 9714940
This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.
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CONTAINERS FOR AGITATION OF LIQUID SAMPLES AND METHODS OF USE THEREOF
Filed US 20170246599
Invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.
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Conduit-containing devices and methods for analyte processing and detection
Issued US 9568575
This invention features devices and methods for analyte processing and detection, and use of such methods, e.g., in the treatment and diagnosis of disease or determining the presence of a pathogen.
Other inventorsSee patent -
Wearable diagnostic platform using direct magnetic detection of magnetic nanoparticles in vivo
Filed US 20160296145
Wearable devices configured to detect the presence, concentration, number, or other properties of magnetic nanoparticles disposed in subsurface vasculature of a person are provided. The wearable devices are configured to detect, using one or more magnetometers, magnetic fields produced by the magnetic nanoparticles. In some embodiments, the magnetometer(s) are atomic magnetometers. In some embodiments, the wearable devices include magnets or other means to magnetize the magnetic nanoparticles…
Wearable devices configured to detect the presence, concentration, number, or other properties of magnetic nanoparticles disposed in subsurface vasculature of a person are provided. The wearable devices are configured to detect, using one or more magnetometers, magnetic fields produced by the magnetic nanoparticles. In some embodiments, the magnetometer(s) are atomic magnetometers. In some embodiments, the wearable devices include magnets or other means to magnetize the magnetic nanoparticles. In some embodiments, the wearable devices produce a time-varying magnetic field, and the magnetometer(s) are configured to detect a time-varying magnetic field responsively produced by the magnetic nanoparticles. In some embodiments, the magnetic nanoparticles are configured to bind to an analyte of interest and detected properties of the magnetic nanoparticles can be used to determine the presence, concentration, or other properties of the analyte.
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Rotating-Frame Gradient Fields for Magnetic Resonance Imaging and Nuclear Magnetic Resonance in Low Fields
Issued US 8633693
A system and method for Fourier encoding a nuclear magnetic resonance (NMR) signal is disclosed. A static magnetic field B0 is provided along a first direction. An NMR signal from the sample is Fourier encoded by applying a rotating-frame gradient field BG superimposed on the B0, where the BG comprises a vector component rotating in a plane perpendicular to the first direction at an angular frequency in a laboratory frame. The Fourier-encoded NMR signal is detected.
Other inventors -
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Rotating-frame gradient fields for magnetic resonance imaging and nuclear magnetic resonance in low fields
Issued US 8633693
A system and method for Fourier encoding a nuclear magnetic resonance (NMR) signal is disclosed. A static magnetic field B0 is provided along a first direction. An NMR signal from the sample is Fourier encoded by applying a rotating-frame gradient field BG superimposed on the B0, where the BG comprises a vector component rotating in a plane perpendicular to the first direction at an angular frequency ω in a laboratory frame. The Fourier-encoded NMR signal is detected.
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NMR, MRI, and spectroscopic MRI in inhomogeneous fields
Issued US 8614575
A method for locally creating effectively homogeneous or “clean” magnetic field gradients (of high uniformity) for imaging (with NMR, MRI, or spectroscopic MRI) both in in-situ and ex-situ systems with high degrees of inhomogeneous field strength.
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NMR Systems and Methods for the Rapid Detection of Analytes
Filed US US 2012/0100546 A1
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NMR, MRI, and Spectroscopic MRI in Inhomogeneous Fields
Filed US US 2010/0102811 A1
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Magnetic Nanoparticle Detection and Separation by Magnetic Relaxation Time
US 20170000375
Wearable devices configured to detect the presence, concentration, number, or other properties of magnetic nanoparticles disposed in subsurface vasculature of a person are provided. The wearable devices are configured to detect, using one or more magnetometers, magnetic fields produced by the magnetic nanoparticles. In some embodiments, the magnetometer(s) are atomic magnetometers. In some embodiments, the wearable devices include magnets or other means to magnetize the magnetic nanoparticles…
Wearable devices configured to detect the presence, concentration, number, or other properties of magnetic nanoparticles disposed in subsurface vasculature of a person are provided. The wearable devices are configured to detect, using one or more magnetometers, magnetic fields produced by the magnetic nanoparticles. In some embodiments, the magnetometer(s) are atomic magnetometers. In some embodiments, the wearable devices include magnets or other means to magnetize the magnetic nanoparticles. In some embodiments, the wearable devices produce a time-varying magnetic field, and the magnetometer(s) are configured to detect a time-varying magnetic field responsively produced by the magnetic nanoparticles. In some embodiments, the magnetic nanoparticles are configured to bind to an analyte of interest and detected properties of the magnetic nanoparticles can be used to determine the presence, concentration, or other properties of the analyte.
Other inventors
Languages
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English
Native or bilingual proficiency
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Greek
Native or bilingual proficiency
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