Brian O'Mara

San Antonio, Texas, United States Contact Info
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Downstream process development scientist in support of protein…

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Experience & Education

  • Scorpius BioManufacturing, Inc.

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Publications

  • Single-stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production

    Biotechnology Progress

    A single-stage clarification was developed using a single-use chromatographic clarification device (CCD) to recover a recombinant protein from Chinese Hamster Ovary (CHO) harvest cell culture fluid (HCCF). Clarification of a CHO HCCF is a complex and costly process, involving multiple stages of centrifugation and/or depth filtration to remove cells and debris and to reduce process-related impurities such as host cell pro- tein (HCP), nucleic acids, and lipids. When using depth filtration, the…

    A single-stage clarification was developed using a single-use chromatographic clarification device (CCD) to recover a recombinant protein from Chinese Hamster Ovary (CHO) harvest cell culture fluid (HCCF). Clarification of a CHO HCCF is a complex and costly process, involving multiple stages of centrifugation and/or depth filtration to remove cells and debris and to reduce process-related impurities such as host cell pro- tein (HCP), nucleic acids, and lipids. When using depth filtration, the filter train consists of multiple filters of varying ratios, layers, pore sizes, and adsorptive properties. The depth filters, in combination with a 0.2-micron membrane filter, clarify the HCCF based on size-exclusion, adsorptive, and charge-based mechanisms, and provide robust bio- burden control. Each stage of the clarification process requires time, labor, and utilities, with product loss at each step. Here, use of the 3MTM Harvest RC Chromatographic Clarifier, a single-stage CCD, is identified as an alternative strategy to a three-stage fil- tration train. The CCD results in less overall filter area, less volume for flushing, and higher yield. Using bioprocess cost modeling, the single-stage clarification process was compared to a three-stage filtration process. By compressing the CHO HCCF clarifica- tion to a single chromatographic stage, the overall cost of the clarification process was reduced by 17%–30%, depending on bioreactor scale. The main drivers for the cost reduction were reduced total filtration area, labor, time, and utilities. The benefits of the single-stage harvest process extended throughout the downstream process, result- ing in a 25% relative increase in cumulative yield with comparable impurity clearance.

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  • Impact of Depth Filtration on Disulfide Bond Reduction during Downstream Processing of Monoclonal Antibodies from CHO Cell Cultures

    Biotechnology and Bioengineering

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  • Development and scale-up of the recovery and purification of a domain antibody Fc fusion protein- comparison of a two and three-step platform approach

    Biotechnology and Bioengineering

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  • Development of a Pareto Optimization Algorithm for the Downstream Process Development of a Monoclonal Antibody

    HTPD International Conference, Avignon, France, June, 2012. (Extended Abstract)

    Other authors
    • Lily Tsang
    • Nicole Payonk
    • Sibylle Herzer
    • Jose Tabora
    • Siegfried Rieble

  • Glycine to glutamic acid misincorporation observed in a recombinant protein expressed by Escherichia coli cells

    Protein Sciences

    Other authors
    • Yunping Huang

  • A Tris (2-Carboxyethyl) Phosphine (TCEP) Related Cleavage on Cysteine-Containing Proteins

    American Society for Mass Spectrometry

    Other authors
    • Peiran Liu

  • Characterization of S-thiolation on secreted proteins from E. coli by mass spectrometry

    Rapid Communications in Mass Spectrometry

    Other authors
    • Peiran Liu

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