Questions tagged [microscopy]
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Does the coherence of light sources make a difference in exciting fluorescence?
Using LED vs laser as light sources in wide-field epi-fluorescence microscopy are both popular choices. It seems that high intensity single color LEDs (such as this one) emits high radiometric power ...
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Electron microscopy and Interatomic distances of miller planes [duplicate]
I am studying Transmission Electron Microscopy (TEM), and have been seeing in articles TEM images of different materials typically come accompanied by these diffraction patterns, caused by the ...
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Can we use prism in fluorescence microscope instead of dichroic mirror?
For the light path outlined here, if we use a prism instead of a dichroic mirror, the excitation light can also pass through the prism, but the emission filter can block the excitation wavelength if ...
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Why is the shorter the wavelength, the smaller the object you can image? and vice-versa?
I know that the shorter the wavelength the smaller the object you can image clearly. Why wavelength matters in imaging something? How having big wavelength wont let u image smaller object, like if u ...
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When to add signals coherently and incoherently?
I just learned in transmission electron microscopy about coherent and incoherent summation. I can't seem to wrap my head around the fact that intensities can be added incoherently. Why can you do that?...
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How to condition an STM tip?
I am starting work on an atomic force microscope and a scanning tunneling microscope and I wanted to ask for advice. How to proceed to get the sharpest tip and therefore the best resolution?
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Limiting factors in traditional microscopy
I have been researching Entanglement-Enhanced Microscopy, and the overall breakthrough seems to be obtaining a resolution of $1/N$ as opposed to $1/\sqrt N$. This led me to ask what the limiting ...
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Image of an electron
Suppose we are trying to see the image of an electron with a microscope. Is it possible? And if it's possible then what do we see? A point at a time, or a blurry spot, or something else?
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Where is the final image located at in a compound microscope?
In a compound microscope, is there a standard as to where the final image should be located? After doing some research, I arrived at either the final image should be located at infinity (to be focused ...
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What is the relation between image resolution and Cutoff from 2D FFT functions in frequency space?
This might be dumb, but unfortunately I need some urgent help about Cutoff from 2D FFT functions in frequency space.
I am writing my bachelor thesis, near DDL and cannot get a lot of help offline in ...
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Simple interference confocal microscope
I have the two articles:
The first one: First article
and
The second one: Second article
After reading the first article I understood while reading that using the detector A we get confocal ...
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Trying to understand how two relay lenses can be used in a light microscope to create a remote Fourier plane for easy access
I am researching the use of relay lens systems to create an additional Fourier plane within a light microscope… to allow easy remote access for filters or masks. I have included some diagrams. One ...
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Why does Near Infrared (NIR) light allow deeper imaging in biological tissue in Multiphoton Microscopy?
I have been reading this paper on multiphoton microscopy (https://www.nature.com/articles/nphoton.an.2010.2) and I am very confused about something. Here is a quote from the paper:
"The NIR ...
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Why transparent object altering only the phase of light but not the frequency or amplitude? Is there exception?
I have learned the fascinating phase contrast microscope from Eugene's textbook optics that made transparent object become visible.
From page 647, he said that
In contradistinction, it is often ...
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Can a system of lenses or compound lens have a higher numerical aperture than a single element in the system?
I am attempting to create a simple Abbe condenser for a microscope, which typically consists of two converging lenses.
Ideally, I would like to achieve a numerical aperture for the condenser of ...
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