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A practical workflow for forensic species identification using direct sequencing of real-time PCR products
BackgroundForensic scientists are often required to identify species of unknown biological samples. Although methods based on sequencing of DNA...
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Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products
Polymerase Chain Reaction (PCR) products have been traditionally characterized by cloning and cycle sequencing. However, when quick sequencing data...
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Penta-Primer Amplification Refractory Mutation System (PARMS) with Direct PCR-Based SNP Marker-Assisted Selection (D-MAS)
The penta-primer amplification refractory mutation system (PARMS) is a high-throughput, low-cost, and automated genotyping assay system that utilizes...
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A multiplex direct PCR method for the rapid and accurate discrimination of three species of spider mites (Acari: Tetranychidae) in fruit orchards in Beijing
Spider mites ( Acari : Tetranychidae ) are polyphagous pests of economic importance in agriculture, among which the two-spotted spider mite Tetranychus...
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Multiplex on-Chip PCR with Direct Detection of Immobilized Primer Elongation
A variant of multiplex PCR on a chip with direct detection of immobilized primer elongation has been developed. Detection is performed by determining...
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QIAGEN’s Investigator® 24plex QS and GO! PCR Amplification
The Investigator® 24Plex kits are multiplex PCR kits utilized by forensic laboratories to simultaneously amplify 22 of the most commonly utilized STR...
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Applied Biosystems’ GlobalFiler™ PCR Amplification Kit
The GlobalFiler™ PCR Amplification Kit is one of the most sensitive kits that exist today that makes the PCR amplification of human DNA possible. PCR...
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DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR
Mycobacterium ulcerans is a slow-growing environmental bacterium that causes a severe skin disease known as Buruli ulcer (BU). Rapid detection of M....
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Rapid and robust squashed spore/colony PCR of industrially important fungi
BackgroundFungi have been utilized for centuries in medical, agricultural, and industrial applications. Development of systems biology techniques has...
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Successful application of modified crude DNA extraction from muscle tissues for various types of PCR amplifications
BackgroundOne of the most challenging aspects of nucleic acid amplification tests is the extraction of genomic DNA. However, achieving satisfactory...
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Real-Time or Quantitative PCR
Real-time PCR is employed for the estimation of differential mRNA expression profile of genes. It is the quantitative expression of a series of genes...
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Direct Detection of SARS-CoV-2 RNA in Saliva with Colorimetric RT-LAMP
RT-LAMP is an effective alternative to RT-PCR-based diagnostics, offering high specificity, sensitivity, and rapid results. One notable advantage is...
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Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions
Emerging viruses pose significant threats to human health and the global economy. In the past two decades, three different coronaviruses have emerged...
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Rapid screening of point mutations by mismatch amplification mutation assay PCR
AbstractMetabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only...
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Timely gene detection assay and reliable screening of genetically engineered plants using an improved direct PCR-based technology
Engineered plants have been widely produced for fundamental and practical use. Several methods have been developed for genetically modified crop...
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Primer extension refractory PCR: an efficient and reliable genome walking method
Genome walking, a molecular technique for obtaining unknown flanking genomic sequences from a known genomic sequence, has been broadly applied to...
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Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia
BackgroundSince the discovery more than half a century ago, cell-free DNA (cfDNA) has become an attractive objective in multiple diagnostic,...
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Usefulness of Quantitative PCR in Forensic Genetics
Although most DNA studies in forensic genetics are focused on the sample, including DNA extraction and amplification, sample quantification is an...
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A Panel of Real-Time PCR Assays for the Direct Detection of All of the Xylella fastidiosa Subspecies
The bacterial plant pathogenPlant pathogen Xylella fastidiosa causes disease in hundreds of plant species worldwide including many crops, and as such...
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Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study
Timely detection of outbreaks is needed for poliovirus eradication, but gold standard detection in the Democratic Republic of the Congo takes 30 days...
