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Fluorescence In Situ Hybridization (FISH) Methods and Protocols
This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically...
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Fish Distant Hybridization
The book introduces fish distant hybridization, which covers the basic theory and applications of fish distant hybridization as well as the main...
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Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue
In situ hybridization is a standard procedure for visualizing mRNA transcripts in tissues. The recent adoption of fluorescent probes and new signal...
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Unequivocal Identification Through Fluorescence In Situ Hybridization (FISH) Technique
Protists represent the largest portion of the diversity of eukaryotes in the globe and have been recognized for performing indispensable activities...
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How common is hybridization in birds?
Hybridization—the interbreeding of different species—plays an integral role in the evolution of numerous bird species. However, it remains unclear...
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Liquid and Solid Hybridization Methods to Detect RNAs
Northern blotting (NB) has been a long-standing method for RNA detection. However, its labor-intensive nature, reliance on high-quality RNA, and use...
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Whole-Mount RNA In Situ Hybridization of Zebrafish Embryos
A commonly employed technique in molecular biology to evaluate the temporal and spatial expression of a certain gene is in situ hybridization. This...
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Thyramide Signal Amplification: New Opportunities for DNA in Situ Hybridization
Abstract —DNA in situ hybridization (ISH) is a valuable technique in molecular cytogenetics for precisely localizing specific DNA sequences on...
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Molecular hybridization strategy for tuning bioactive peptide function
The physicochemical and structural properties of antimicrobial peptides (AMPs) determine their mechanism of action and biological function. However,...
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Formamide-Free Genomic In Situ Hybridization (ff-GISH)
Fluorescence in situ hybridization allows for the mapping of various sequence types in the genomes and is thus widely used in structural, functional,...
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Biofortification of Legume Hybrids Obtained Through Intergeneric Hybridization
Agricultural biotechnology has allowed scientists to introduce novel features into commonly consumed staple crops to enhance their productivity. One...
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RNA Double-Helix Hybridization Measured by Fluorescence Correlation Spectroscopy
RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base...
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Inconsistent estimates of hybridization frequency in newts revealed by SNPs and microsatellites
Hybridization between the European smooth and palmate newts has recurrently been mentioned in the literature. The only two studies that attempted to...
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Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or...
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Shallow sequencing can mislead when evaluating hybridization capture methods
Hybridization capture (also called enrichment) is a molecular technique used to focus high-throughput sequencing effort to a targeted region (or...
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Gene Expression Patterns in the Ctenophore Pleurobrachia bachei: In Situ Hybridization
In situ hybridization is a powerful and precise tool for revealing cell- and tissue-specific gene expression and a critical approach to validating...
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Development of an 8K SNP chip to assess adaptive diversity and hybridization in polar bears
The polar bear ( Ursus maritimus ) is a species particularly vulnerable to the effects of climate change. As the climate warms, polar bears will be...
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Visualization of Individual RNA Molecules by Proximity Ligation-Based Chromogenic In Situ Hybridization Assay
RNA in situ hybridization reveals the abundance and location of gene expression in cells or tissues, providing a technical basis for the clinical...
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Cytogenetic analysis reveals a mechanism of compatibility in distant hybridization between tree peony and herbaceous peony
Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies ( Paeonia sect. Moutan ) and herbaceous...
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Turning the threat of hybridization into an asset for conserving wild species by backcrossing
Captive breeding programs working to avoid the loss of threatened wild species are often working with a limited gene pool of founder individuals...
