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Optimization of long-range PCR protocol to prepare filaggrin exon 3 libraries for PacBio long-read sequencing
BackgroundThe filaggrin (FLG) protein, encoded by the FLG gene, is an intermediate filament-associated protein that plays a crucial role in the...
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Whole-Genome Sequencing of Plants: Past, Present, and Future
Advances in analytical chemistry, as well as the constant introduction of novel analytical chemistry approaches, are altering genomics at a higher...
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L-GIREMI uncovers RNA editing sites in long-read RNA-seq
Although long-read RNA-seq is increasingly applied to characterize full-length transcripts it can also enable detection of nucleotide variants, such...
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Whole-genome sequencing and analysis of Chryseobacterium arthrosphaerae from Rana nigromaculata
Chryseobacterium arthrosphaerae strain FS91703 was isolated from Rana nigromaculata in our previous study. To investigate the genomic...
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Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey
AbstractThe aim of this study was the whole-genome analysis and assessment of the antimicrobial potential of bacterial isolates from honey harvested...
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Broad Genome Sequencing of Environmental and Clinical Strains and Genotyping
The Next-Generation Sequencing revolution had a great impact on the genomics of Pseudomonas aeruginosa. Since the first release of the P. aeruginosa...
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Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy
BackgroundThe mining of new allelic variation and the induction of new genetic variability are the basis for improving breeding efficiency.
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A random priming amplification method for whole genome sequencing of SARS-CoV-2 virus
BackgroundNon-targeted whole genome sequencing is a powerful tool to comprehensively identify constituents of microbial communities in a sample....
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The optimal standard protocols for whole-genome sequencing of antibiotic-resistant pathogenic bacteria using third-generation sequencing platforms
BackgroundThe emergence of antibiotic-resistant bacterial pathogens in the environment has been increasing, posing a threat to public health....
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Whole-Genome Bisulfite Sequencing for Detection of DNA Methylation in Crops
DNA methylations are well known for their regulatory functions and involved in various biological processes. Due to its great importance, the...
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Whole-genome sequencing and comparative genomics reveal candidate genes associated with quality traits in Dioscorea alata
BackgroundQuality traits are essential determinants of consumer preferences. Dioscorea alata (Greater Yam), is a starchy tuber crop in tropical...
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A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice
Efficient, accurate molecular characterization of genetically modified (GM) organisms is challenging, especially for those transgenic events...
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vSNP: a SNP pipeline for the generation of transparent SNP matrices and phylogenetic trees from whole genome sequencing data sets
BackgroundSeveral single nucleotide polymorphism (SNP) pipelines exist, each offering its own advantages. Among them and described here is vSNP that...
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Lerna: transformer architectures for configuring error correction tools for short- and long-read genome sequencing
BackgroundSequencing technologies are prone to errors, making error correction (EC) necessary for downstream applications. EC tools need to be...
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Whole-genome sequencing analysis of two heat-evolved Escherichia coli strains
BackgroundHigh temperatures cause a suite of problems for cells, including protein unfolding and aggregation; increased membrane fluidity; and...
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Long-read sequencing settings for efficient structural variation detection based on comprehensive evaluation
BackgroundWith the rapid development of long-read sequencing technologies, it is possible to reveal the full spectrum of genetic structural variation...
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The use of long-read PCR amplicon sequencing to study the evolution of resistance to zoxamide, oxathiapiprolin and complex III inhibitors in French Plasmopara viticola field populations
Molecular methods are one of the most effective tools to monitor fungicide resistance. Long-read sequencing is an emerging technology in the field of...
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Technologies Promoting Genome-Based Taxonomy
Whole-genome sequencing (WGS) has proven to be a reliable method. Additionally, it is a commonly used approach in research and surveillance...
