Abstract
Photothrombosis is one of the techniques available to reproduce ischemic injuries in animal models. Most of the studies that use photothrombosis resort to this technique as it is highly reproducible and minimally invasive to target cortical brain regions, such as the motor or somatosensory areas. However, this technique can be modified and adapted to virtually target any brain region, including deeper tissue. Here, we describe some variations on the traditional protocol to use the photothrombotic technique to target the longitudinal hippocampal vein in the adult mouse and cause an ischemic injury in the hippocampus.
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References
Al Shoyaib A, Alamri FF, Biggers A et al (2021) Delayed exercise-induced upregulation of Angiogenic proteins and recovery of motor function after photothrombotic stroke in mice. Neuroscience 461:57–71
Barth AM, Mody I (2011) Changes in hippocampal neuronal activity during and after unilateral selective hippocampal ischemia in vivo. J Neurosci 31:851–860
Bederson JB, Pitts LH, Germano SM et al (1986) Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke 17:1304–1308
Kuroiwa T, Xi G, Hua Y et al (2009) Development of a rat model of photothrombotic ischemia and infarction within the caudoputamen. Stroke 40:248–253
Labat-Gest V, Tomasi S (2013) Photothrombotic ischemia: a minimally invasive and reproducible photochemical cortical lesion model for mouse stroke studies. J Vis Exp 76:e50370
Liang H, Zhao H, Gleichman A et al (2019) Region-specific and activity-dependent regulation of SVZ neurogenesis and recovery after stroke. Proc Natl Acad Sci 116:13621
Rosenblum WI, El-Sabban F (1977) Platelet aggregation in the cerebral microcirculation: effect of aspirin and other agents. Circ Res 40:320–328
Watson BD, Dietrich WD, Busto R et al (1985) Induction of reproducible brain infarction by photochemically initiated thrombosis. Ann Neurol 17:497–504
Zeiger WA, Marosi M, Saggi S et al (2021) Barrel cortex plasticity after photothrombotic stroke involves potentiating responses of pre-existing circuits but not functional remapping to new circuits. Nat Commun 12:3972
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Blanco-Suárez, E. (2023). Photothrombotic Model to Create an Infarct in the Hippocampus. In: Karamyan, V.T., Stowe, A.M. (eds) Neural Repair. Methods in Molecular Biology, vol 2616. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2926-0_4
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DOI: https://doi.org/10.1007/978-1-0716-2926-0_4
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2926-0
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