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The Photothrombotic Model of Ischemic Stroke

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Neurobiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2746))

Abstract

Stroke is a major cause of morbidity worldwide; yet, there is a lack of treatment options to address post-stroke cognitive and motor impairment, thus there is an urgency for developing neuroprotective and restorative therapies. Much of our fundamental understanding of stroke pathology has been derived from animal models. The photothrombotic model of ischemic stroke is commonly used to study cellular and molecular mechanisms of neurodegeneration, test functional/cognitive outcomes, identify important biomarkers, and assess the effectiveness of novel therapies. It allows for the precise targeting of an infarct to a specific region of the brain, has a low mortality rate, low seizure rate, and is relatively easy to perform. This chapter outlines materials and methods for the photothrombotic model of ischemic stroke in mice, its limitations, and some considerations needed when using this model.

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Authors and Affiliations

  1. Centre for Cardiovascular Biology and Disease Research, Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, VIC, Australia

    David E. Wong Zhang, Shenpeng R. Zhang, Hyun Ah. Kim, Christopher G. Sobey & T. Michael De Silva

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  1. David E. Wong Zhang
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  2. Shenpeng R. Zhang
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  3. Hyun Ah. Kim
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  4. Christopher G. Sobey
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  5. T. Michael De Silva
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Correspondence to T. Michael De Silva .

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  1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, VIC, Australia

    Sebastian Dworkin

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Wong Zhang, D.E., Zhang, S.R., Kim, H.A., Sobey, C.G., De Silva, T.M. (2024). The Photothrombotic Model of Ischemic Stroke. In: Dworkin, S. (eds) Neurobiology. Methods in Molecular Biology, vol 2746. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3585-8_18

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  • DOI: https://doi.org/10.1007/978-1-0716-3585-8_18

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