The Nextflow extension package for Google Cloud is a plugin provided by Seqera Labs that allows the support for Google Filestore file system when deploying Nextflow pipelines with Google LifeSciences and Google Cloud Batch computing services.

The plugin requires a license key to be used. If you are interested, please contact us for an evaluation license at sales@seqera.io.

• Java 11 or later
• Nextflow 23.01.0-edge or later
• Google LifeScience or Google Batch API enabled
• Google Filestore instance

• Install Java and the required NFS system dependecies

  sudo apt-get -y install openjdk-11-jdk-headless nfs-common
  

• Install Nextflow:

  export NXF_VER=23.01.0-edge
  curl get.nextflow.io | bash
  ./nextflow self-update
  

• Configure the Google crendentials defining the variable GOOGLE_APPLICATION_CREDENTIALS in the launching environment, e.g.:

  export GOOGLE_APPLICATION_CREDENTIALS=$PWD/google.json
  

• Mount the shared file system in the launching environment:

  sudo mkdir /nfs
  sudo mount 10.195.15.250:/share1 /nfs/
  sudo chmod  go+rw /nfs
  

  In the above snippet replace the 10.195.15.250:/share1 string with your Filestore instance mount point. See the Google Filestore documentation for details.

Configure the Nextflow XPACK license definining the variable NXF_XPACK_LICENSE in the launching environment, e.g.:

export NXF_XPACK_LICENSE=<license string>

Create the nextflow.config file in the pipeline launching directory with the following settings:

plugins {
  id 'xpack-google@1.0.0'
}

google.location = 'europe-west2'
google.batch.nfsVolumes.nfs1.target = '10.195.15.250:/share1'
google.batch.nfsVolumes.nfs1.mountPath = '/nfs'

process.executor = 'google-batch' 

In the above example replace the region, NFS target and mountPath with the values matching your Filestore instance configuration.

Note: more than one Filestore instance can be configured providing the corresponding target and mountPath values using a unique nfsVolumes names for each of them e.g. nfs1, nfs2, etc.

Create the nextflow.config file in the pipeline launching directory with the following settings:

plugins {
  id 'xpack-google@1.0.0'
}

google.region = 'europe-west2'
google.lifeSciences.nfsVolumes.nfs1.target = '10.195.15.250:/share1'
google.lifeSciences.nfsVolumes.nfs1.mountPath = '/nfs'

process.executor = 'google-lifesciences' 

In the above example replace the region, NFS target and mountPath with the values matching your Filestore instance configuration.

Note: more than one Filestore instance can be configured providing the corresponding target and mountPath values using a unique nfsVolumes names for each of them e.g. nfs1, nfs2, etc.

Launch the Nextflow pipeline execution specifying the as work directory a path in the shared file system, e.g.:

./nextflow run hello -w /nfs/scratch

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