Sex-Dependent Expression of Caveolin 1 in Response to Sex Steroid Hormones Is Closely Associated with Development of Obesity in Rats
Figure 6
Representative silver-stained SDS-PAGE images of co-immunoprecipitated samples of abdominal WAT and BAT (M: maker, C: control, E2: E2-treated, DHT: DHT-treated, PC: positive control, T: total protein).
Proteins were identified by PMF and indicated by black arrows along with their abbreviated names. AMIB: N-acetylmuramoyl-L-alanine amidase; CAR3: carbonic anhydrase 3; ACO2: aconitate hydratase, mitochondrial precursor; ATP5B: ATP synthase (H+ transporting, mitochondrial F1 complex, beta polypeptide); ACAA2: acetyl-CoA acyltransferase 2, mitochondrial; GAPDH: glycerol 3-phosphate dehydrogenase; ETF: electron transfer flavoprotein, subunit beta (ETF); TPI1: triose phosphate isomerase 1 (A). Protein levels of two major mitochondrial proteins, ETF and ATP5B, were identified by PMF in abdominal WAT and BAT (B). Band densities were normalized using β-actin in each tissue to calculate relative intensity (%). Statistical significance between control and hormone treated groups were calculated by Student’s t-test, where, *p<0.05 and **p<0.01. The significance of the effects of sex, diet and hormonal treatments was tested using multivariate ANOVA (M-ANOVA), where p<0.05.
