CD8A, CPTAC-6226 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CD8A	MAL

Sequence Length (AA)	Molecular Weight (Da)
235	25729

Protein Name
T-cell surface glycoprotein CD8 alpha chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MALPVTALLL	PLALLLHAAR	PSQFRVSPLD	RTWNLGETVE	LKCQVLLSNP
60	70	80	90	100
TSGCSWLFQP	RGAAASPTFL	LYLSQNKPKA	AEGLDTQRFS	GKRLGDTFVL
110	120	130	140	150
TLSDFRRENE	GYYFCSALSN	SIMYFSHFVP	VFLPAKPTTT	PAPRPPTPAP
160	170	180	190	200
TIASQPLSLR	PEACRPAAGG	AVHTRGLDFA	CDIYIWAPLA	GTCGVLLLSL
210	220	230	235
VITLYCNHRN	RRRVCKCPRP	VVKSGDKPSL	SARYV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6226 Collapse assay details

Data source: Panorama

Official Gene Symbol: CD8A
Peptide Sequence: TWNLGETVELK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 32
Peptide End: 42

CPTAC ID: CPTAC-6226
Peptide Molecular Mass: 1,288.6663
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	14.7	12.5	5	9.6	8.7	12.7	17.6	15.2	13.6	15	15	15
y9 (1+)	18.8	16.6	5.6	6.7	8.9	9.4	20	18.8	10.9	15	15	15
y7 (1+)	13	17.5	5.6	7	7.8	7.1	14.8	19.2	9	15	15	15
b3 (1+)	17	14.3	4	10.6	11.8	9.7	20	18.5	10.5	15	15	15
sum	15.4	15.8	4.5	5	8.3	8.6	16.2	17.8	9.7	15	15	15

CD8A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6226 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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