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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SLAMF7 CS1
Sequence Length (AA) Molecular Weight (Da)
335 37421
Protein Name
SLAM family member 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGSPTCLTL IYILWQLTGS AASGPVKELV GSVGGAVTFP LKSKVKQVDS
60 70 80 90 100
IVWTFNTTPL VTIQPEGGTI IVTQNRNRER VDFPDGGYSL KLSKLKKNDS
110 120 130 140 150
GIYYVGIYSS SLQQPSTQEY VLHVYEHLSK PKVTMGLQSN KNGTCVTNLT
160 170 180 190 200
CCMEHGEEDV IYTWKALGQA ANESHNGSIL PISWRWGESD MTFICVARNP
210 220 230 240 250
VSRNFSSPIL ARKLCEGAAD DPDSSMVLLC LLLVPLLLSL FVLGLFLWFL
260 270 280 290 300
KRERQEEYIE EKKRVDICRE TPNICPHSGE NTEYDTIPHT NRTILKEDPA
310 320 330 335
NTVYSTVEIP KKMENPHSLL TMPDTPRLFA YENVI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1763 Collapse assay details

Data source: Panorama

Official Gene Symbol
SLAMF7
Peptide Sequence
VDFPDGGYSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
81
Peptide End
91
CPTAC ID
CPTAC-1763
Peptide Molecular Mass
1,196.5714
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 6.3 4.5 1.8 7.5 5.5 1.9 9.8 7.1 2.6 15 15 15
y7 (1+) 8.8 2.4 2 10.4 3.3 2.1 13.6 4.1 2.9 15 15 15
y8 (1+) 2.6 3.6 2 4 3.2 2.1 4.8 4.8 2.9 15 15 15
sum 3.5 3.1 1 3.9 3.4 1.1 5.2 4.6 1.5 15 15 15


Additional Resources and Comments