Background
Catalog Number:
CPTC-CD8A-1
RRID:
AB_2890079
Target Antigen:
CD8A Molecule Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Endogenous
External Links
- CPTAC Assay Portal CPTC-CD8A-1
Purchase
- DSHB CPTC-CD8A-1
Characterization Data [Compare Characterization Data]
Immuno-MRM
Click image to enlarge
CPTC-CD8A-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-CD8A-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6226)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-CD8A-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-CD8A-1 Western Blot (Over-Expressed Lysate)
Result: Negative
Western blot using CPTC-CD8A-1 as primary antibody against transient CD8A overexpression lysate. The expected molecular weight is 25.7 kDa.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
Click image to enlarge
CPTC-CD8A-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-CD8A-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 25.7 kDa, 21.6 kDa, and 30.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
Click image to enlarge
CPTC-CD8A-1 Automated Western Blot (Cell Lysate)
Result: Negative
Automated western blot using CPTC-CD8A-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 25.7 kDa, 21.6 kDa, and 30.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CD8A-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-CD8A-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 25.7 kDa. Cytochrome C was used as a loading control.
Characterization SOP Files
Background
NCI Identification Number:
00359
Antigen Name:
CD8A Molecule Peptide 1
CPTC Name:
CPTC-CD8A Peptide 1
Aliases:
CD8a Molecule; T-Cell Surface Glycoprotein CD8 Alpha Chain; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); Leu2 T-Lymphocyte Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor; T8 T-Cell Antigen; CD8a Antigen; Leu2; CD8A; CD8; P32; MAL
Function:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. CD8A (CD8a Molecule) is a Protein Coding gene. Diseases associated with CD8A include Cd8 Deficiency, Familial and Herpes Zoster. Among its related pathways are TCR Signaling (Qiagen) and T cell receptor signaling pathway. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and coreceptor activity.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
Chromosomal Localization:
2p11.2
Accession Number:
NP_001369627.1
UniProt Accession Number:
P01732
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
TWNLGETVELK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
09/17/2020
Links
- EnsEMBL - EnsEMBL CD8A
- Entrez - Entrez CD8A
- Uniprot - Uniprot P01732
Characterization Data
SOPs
No SOPs available.
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