Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anticancer Immunity
- PMID: 29764863
- DOI: 10.1158/0008-5472.CAN-18-0171
Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anticancer Immunity
Abstract
Extracorporeal photochemotherapy (ECP) is a cancer immunotherapy for cutaneous T-cell lymphoma (CTCL) operative in more than 350 centers worldwide. Although its efficacy and favorable safety profile have driven its widespread use, elucidation of its underlying mechanism has been difficult. In this study, we identify the principal contributors to the anticancer immunotherapeutic effects of ECP, with the goal of enhancing potency and broadening applicability to additional malignancies. First, we scaled down the clinical ECP leukocyte-processing device to mouse size. Second, we used that miniaturized device to produce a cellular vaccine that regularly initiated therapeutic antimelanoma immunity. Third, we individually subtracted key factors from either the immunizing inoculum or the treated animal to ascertain their contribution to the in vivo antimelanoma response. Platelet-signaled monocyte-to-dendritic cell (DC) differentiation followed by sorting/processing/presentation of tumor antigens derived from internalized apoptotic tumor cells were absolute requirements. As in clinical ECP, immunogenic cell death of tumor cells was finely titrated by DNA cross-linkage mediated by photoactivated 8-methoxypsoralen (8-MOPA). ECP-induced tumor-loaded DC were effective immunotherapeutic agents only if they were spared exposure to 8-MOPA, indicating that healthy DC are required for ECP. Infusion of responder T cells into naïve tumor-challenged mice established the protective role of stimulated T-cell antitumor immunity. Collectively, these results reveal that selective antitumor effects of ECP are initiated by tumor antigen-loaded, ECP-induced DC, which promote potent collaboration between CD4 and CD8 tumor-specific T cells. These mechanistic insights suggest potential therapeutic applicability of ECP to solid tumors in addition to CTCL.Significance: These findings identify principal cellular contributors to the anticancer immunotherapeutic impact of ECP and suggest this treatment may be applicable to a broad spectrum of immunogenic malignancies. Cancer Res; 78(14); 4045-58. ©2018 AACR.
©2018 American Association for Cancer Research.
Similar articles
-
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells.J Vis Exp. 2019 May 17;(147). doi: 10.3791/59370. J Vis Exp. 2019. PMID: 31157760
-
Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy.Methods Mol Biol. 2020;2097:173-195. doi: 10.1007/978-1-0716-0203-4_11. Methods Mol Biol. 2020. PMID: 31776926
-
Extracorporeal photochemotherapy induces bona fide immunogenic cell death.Cell Death Dis. 2019 Aug 2;10(8):578. doi: 10.1038/s41419-019-1819-3. Cell Death Dis. 2019. PMID: 31371700 Free PMC article.
-
Efficient tumor antigen loading of dendritic antigen presenting cells by transimmunization.Technol Cancer Res Treat. 2002 Feb;1(1):65-9. doi: 10.1177/153303460200100109. Technol Cancer Res Treat. 2002. PMID: 12614179 Review.
-
Extracorporeal photochemoimmunotherapy in cutaneous T cell lymphomas.Ann N Y Acad Sci. 2001 Sep;941:123-38. doi: 10.1111/j.1749-6632.2001.tb03716.x. Ann N Y Acad Sci. 2001. PMID: 11594566 Review.
Cited by
-
Integration of Single-Cell RNA Sequencing and Bulk RNA Sequencing to Identify an Immunogenic Cell Death-Related 5-Gene Prognostic Signature in Hepatocellular Carcinoma.J Hepatocell Carcinoma. 2024 May 16;11:879-900. doi: 10.2147/JHC.S449419. eCollection 2024. J Hepatocell Carcinoma. 2024. PMID: 38770169 Free PMC article.
-
Identification of immunogenic cell death-related damage-related molecular patterns (DAMPs) to predict outcomes in patients with head and neck squamous cell carcinoma.J Cancer Res Clin Oncol. 2024 May 7;150(5):240. doi: 10.1007/s00432-024-05779-2. J Cancer Res Clin Oncol. 2024. PMID: 38713284 Free PMC article.
-
Targeting immunogenic cell stress and death for cancer therapy.Nat Rev Drug Discov. 2024 Jun;23(6):445-460. doi: 10.1038/s41573-024-00920-9. Epub 2024 Apr 15. Nat Rev Drug Discov. 2024. PMID: 38622310 Free PMC article. Review.
-
Targeting YAP1 to improve the efficacy of immune checkpoint inhibitors in liver cancer: mechanism and strategy.Front Immunol. 2024 Mar 14;15:1377722. doi: 10.3389/fimmu.2024.1377722. eCollection 2024. Front Immunol. 2024. PMID: 38550587 Free PMC article. Review.
-
The effect of UVA light/8-methoxypsoralen exposure used in Extracorporeal Photopheresis treatment on platelets and extracellular vesicles.PLoS One. 2024 Feb 28;19(2):e0293687. doi: 10.1371/journal.pone.0293687. eCollection 2024. PLoS One. 2024. PMID: 38416722 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials