Kondapalli C, et al. (2012) PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65. Open Biol 2, 120080 22724072

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S65-p - PARK2 (human) ▲

Modsite: NCDLDQQsIVHIVQR SwissProt Entrez-Gene Orthologous residues PARK2 (human): S65‑p, PARK2 iso5 (human): S65‑p, PARK2 (mouse): S65‑p, PARK2 (rat): S65‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PINK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PINK1 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CCCP increase siRNA CCCP inhibit treatment-induced increase siRNA PINK1 FCCP increase valinomycin increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S131-p - PARK2 (human) ▲
Modsite: HTDsRkDsPPAGsPA SwissProt Entrez-Gene Orthologous residues PARK2 (human): S131‑p, PARK2 iso5 (human): S131‑p, PARK2 (mouse): S131‑p, PARK2 (rat): S131‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human

T257-p - PINK1 (human) ▲

Modsite: AGEYGAVtYRKSkRG SwissProt Entrez-Gene Orthologous residues PINK1 (human): T257‑p, PINK1 (mouse): T256‑p, PINK1 (rat): T256‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PINK1 (human) transfection of inactive enzyme, pharmacological activator of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CCCP increase