High-Plex Assessment of Biomarkers in Tumors
- PMID: 38219953
- DOI: 10.1016/j.modpat.2024.100425
High-Plex Assessment of Biomarkers in Tumors
Abstract
The assessment of biomarkers plays a critical role in the diagnosis and treatment of many cancers. Biomarkers not only provide diagnostic, prognostic, or predictive information but also can act as effective targets for new pharmaceutical therapies. As the utility of biomarkers increases, it becomes more important to utilize accurate and efficient methods for biomarker discovery and, ultimately, clinical assessment. High-plex imaging studies, defined here as assessment of 8 or more biomarkers on a single slide, have become the method of choice for biomarker discovery and assessment of biomarker spatial context. In this review, we discuss methods of measuring biomarkers in slide-mounted tissue samples, detail the various high-plex methods that allow for the simultaneous assessment of multiple biomarkers in situ, and describe the impact of high-plex biomarker assessment on the future of anatomic pathology.
Keywords: antibody; high-plex; immunofluorescence; immunohistochemistry; spatial.
Copyright © 2024 United States & Canadian Academy of Pathology. Published by Elsevier Inc. All rights reserved.
Similar articles
-
Whole-Slide Imaging, Mutual Information Registration for Multiplex Immunohistochemistry and Immunofluorescence.Lab Invest. 2023 Aug;103(8):100175. doi: 10.1016/j.labinv.2023.100175. Epub 2023 May 15. Lab Invest. 2023. PMID: 37196983
-
Challenges and Opportunities for Immunoprofiling Using a Spatial High-Plex Technology: The NanoString GeoMx® Digital Spatial Profiler.Front Oncol. 2022 Jun 29;12:890410. doi: 10.3389/fonc.2022.890410. eCollection 2022. Front Oncol. 2022. PMID: 35847846 Free PMC article. Review.
-
A new tetra-plex fluorimetric assay for the quantification of cerebrospinal fluid β-amyloid42, total-tau, phospho-tau and α-synuclein in the differential diagnosis of neurodegenerative dementia.J Neurol. 2020 Sep;267(9):2567-2581. doi: 10.1007/s00415-020-09870-9. Epub 2020 May 5. J Neurol. 2020. PMID: 32372181
-
HGF/MET pathway aberrations as diagnostic, prognostic, and predictive biomarkers in human cancers.Crit Rev Clin Lab Sci. 2019 Dec;56(8):533-566. doi: 10.1080/10408363.2019.1653821. Epub 2019 Sep 12. Crit Rev Clin Lab Sci. 2019. PMID: 31512514 Review.
-
High-Plex Spatially Resolved RNA and Protein Detection Using Digital Spatial Profiling: A Technology Designed for Immuno-oncology Biomarker Discovery and Translational Research.Methods Mol Biol. 2020;2055:563-583. doi: 10.1007/978-1-4939-9773-2_25. Methods Mol Biol. 2020. PMID: 31502169
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials