We present a developmental atlas that offers insight into sequential epigenetic changes underlying early human brain development modeled in organoids, which reconstructs the differentiation trajectories of all major CNS regions. It shows that epigenetic regulation via the installation of activating histone marks precedes activation of groups of neuronal genes.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Millan-Zambrano, G. et al. Histone post-translational modifications — cause and consequence of genome function. Nat. Rev. Genet. 23, 563–580 (2022). This review article summarizes how histone modifications contribute to genome functional regulation.
Kelava, I. & Lancaster, M. Dishing out mini-brains: current progress and future prospects in brain organoid research. Dev. Biol. 420, 199–209 (2016). Review article presents how brain organoids are derived and develop in vitro.
Kaya-Okur, H. S. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat. Comm. 10, 1930 (2019). This paper reports the establishment and use of CUT&Tag to record histone modifications.
Wang, H. et al. H3K4me3 regulates RNA polymerase II promoter-proximal pause-release. Nature 615, 339–348 (2023). This paper describes how H3K4me3 influences transcriptional regulation.
Policarpi, C. et al. Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications. Preprint at bioRxiv https://doi.org/10.1101/2022.09.04.506519 (2022). This non-peer-reviewed preprint reports on the effect of histone modifications on regulation transcription in an inert genomic location.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Zenk, F. et al. Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems. Nat. Neurosci. https://doi.org/10.1038/s41593-024-01652-0 (2024).
Rights and permissions
About this article
Cite this article
Dissecting the epigenetic regulation of human brain organoid development in single cells. Nat Neurosci (2024). https://doi.org/10.1038/s41593-024-01653-z
Published:
DOI: https://doi.org/10.1038/s41593-024-01653-z
