We demonstrate CRISPRdelight, a robust CRISPR–Cas12a-based method for imaging non-repetitive genomic DNAs in a highly efficient way. This system is a powerful tool for studying functional links between gene dynamics, localization and regulation, and reveals heterogeneity in the expression of differently localized alleles in the same cells.
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References
Van Tricht, C., Voet, T., Lammertyn, J. & Spasic, D. Imaging the unimaginable: leveraging signal generation of CRISPR-Cas for sensitive genome imaging. Trends Biotechnol. 41, 769–784 (2023). This review article summarizes the available tools for live-cell DNA imaging.
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This is a summary of: Yang, L.-Z. et al. CRISPR-array-mediated imaging of non-repetitive and multiplex genomic loci in living cells. Nat. Methods https://doi.org/10.1038/s41592-024-02333-3 (2024).
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CRISPRdelight illuminates non-repetitive genomic loci in living cells. Nat Methods (2024). https://doi.org/10.1038/s41592-024-02352-0
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DOI: https://doi.org/10.1038/s41592-024-02352-0
