Extended Data Fig. 5: Direct RNA detection in a wFDCF CRISPR-Cas13a based sensor.

A CRISPR-Cas13a based MRSA SHERLOCK RNA sensor was prepared and freeze-dried over a wearable textile device for testing. All reactions contained RNaseAlert substrate, a quenched fluorophore probe that is cleaved by activated Cas13a (Integrated DNA Technologies, Coralville, IA). The wearable sensor was activated with a fluid splash of dd-H₂O containing 20 nM mecA RNA transcript trigger, while the plate samples were rehydrated with the same trigger concentrations to the originally deposited reaction volume (4 μL). Reactions in the wFDCF were monitored at 30 °C for 30 minutes using the wearable optical device and the reference control in a BioTek NEO HTS plate reader (BioTek Instruments, Inc., Winooski, VT) in fluorescence mode (Ex. 470 nm / Em. 528 nm). Normalized pixel intensity in the wearable device is shown as mean (green dark line) ± s.d. (green light region) of n = 3 independent experiments and is comparable in dynamics to the results of the kinetic run conducted in the plate reader (red line) shown as a singlicate reference.