Extended Data Fig. 10: Biophysical characterization of the active state GLFs containing G-protein binding sites. | Nature

Extended Data Fig. 10: Biophysical characterization of the active state GLFs containing G-protein binding sites.

From: Computational design of soluble and functional membrane protein analogues

Extended Data Fig. 10

a, Cartoon depiction of design (colored) overlaid on the target fold (gray). The point mutants of the aGLF (orange) show a zoom with the point mutation (red) in the presence of mini-gs (green). b, SEC-MALS analysis of corresponding design in panel a. The expected Mw for the monomeric design ranges from 33.1 to 34.1 kDa. c, SPR sensorgram of different MiniGs concentrations in the presence of the designs from panel a.

Back to article page