Cross-Reactivity of Ragweed Pollen Calcium-Binding Proteins and IgE Sensitization in a Ragweed-Allergic Population from Western Romania
Abstract
:Highlights
- An IgE reactivity to ragweed pollen calcium-binding proteins (polcalcins) of 25% to rAmb a 9 and 35% to rAmb a 10 was found in a ragweed-allergic population from Romania;
- A strong cross-reactive behavior of rAmb a 9 and rAmb a 10 with rArt v 5 was found, and patients positive to ragweed polcalcins tended to report rather severe respiratory symptoms.
- The higher IgE sensitization rate to ragweed polcalcins in western Romania may be due to heavily invaded areas with ragweed as well as due to polcalcin cross-reactivity;
- Recombinant allergens are a useful tool to detect polcalcin sensitization in ragweed-allergic patients, and ragweed polcalcins may be considered for inclusion in the molecular diagnosis.
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients and Patients’ Sera
2.2. Expression of the Recombinant Polcalcins and Extract Preparation
2.3. IgE Reactivity towards Recombinant Amb a 9 and Amb a 10 in Ragweed-Allergic Patients
2.4. Induction of Allergen-Specific IgG Response in Rabbits
2.5. Detection of Potential Cross-Reactivity towards Ragweed and Mugwort Polcalcins and Extracts in ELISA
2.6. β-Hexosaminidase Release Assay Using Human Rat Basophil Leukemia Cells
2.7. Statistical Analysis
3. Results
3.1. Patients’ Clinical Characterization
3.2. Detection of Specific IgE to rAmb a 9 and rAmb a 10
3.3. Allergenic Activity of rAmb a 9 and rAmb a 10 in Basophil Activation Test
3.4. Association of the Clinical Symptoms with the IgE Response to Ragweed Polcalcins
3.5. Detection of rAmb a 9- and rAmb a 10-Specific IgG Antibodies in Rabbit Serum and Reactivity to Weed Pollen Extracts and Polcalcins
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patients, no. | 87 |
Sex male/female, no. | 48/39 |
Age range (median) | 18–64 (34) |
Prevalence of symptoms (%) | |
Symptoms of rhinitis | 100 |
Symptoms of conjunctivitis | 99 |
Asthma-like symptoms | 67 |
Skin symptoms | 22 |
Sensitized only to ragweed pollen (%) | 34 |
Other sensitizations (%) | |
Grass pollen | 39 |
House dust mites | 30 |
Mugwort pollen | 21 |
Tree pollen | 17 |
Animal dander (cat and dog) | 11 |
Fungi | 6 |
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Zbîrcea, L.-E.; Buzan, M.-R.; Grijincu, M.; Tamaș, T.-P.; Vulpe, C.B.; Huțu, I.; Păunescu, V.; Panaitescu, C.; Chen, K.-W. Cross-Reactivity of Ragweed Pollen Calcium-Binding Proteins and IgE Sensitization in a Ragweed-Allergic Population from Western Romania. Adv. Respir. Med. 2024, 92, 218-229. https://doi.org/10.3390/arm92030022
Zbîrcea L-E, Buzan M-R, Grijincu M, Tamaș T-P, Vulpe CB, Huțu I, Păunescu V, Panaitescu C, Chen K-W. Cross-Reactivity of Ragweed Pollen Calcium-Binding Proteins and IgE Sensitization in a Ragweed-Allergic Population from Western Romania. Advances in Respiratory Medicine. 2024; 92(3):218-229. https://doi.org/10.3390/arm92030022
Chicago/Turabian StyleZbîrcea, Lauriana-Eunice, Maria-Roxana Buzan, Manuela Grijincu, Tudor-Paul Tamaș, Constantina Bianca Vulpe, Ioan Huțu, Virgil Păunescu, Carmen Panaitescu, and Kuan-Wei Chen. 2024. "Cross-Reactivity of Ragweed Pollen Calcium-Binding Proteins and IgE Sensitization in a Ragweed-Allergic Population from Western Romania" Advances in Respiratory Medicine 92, no. 3: 218-229. https://doi.org/10.3390/arm92030022