Scientist I-II Antibody Display

We are hiring a full-time Scientist to join Mosaic’s Antibody Discovery and Optimization team in Boulder, Colorado. The successful candidate will participate in the construction, validation, and screening of surface-displayed antibody libraries. The ideal candidate will have in-depth, hands-on expertise in all steps of display technology (yeast or phage), including DNA library construction, FACS, ELISA, and NGS.

Responsibilities:

• Design and execute antibody discovery and affinity maturation campaigns using yeast and phage display
• Construct libraries using PCR, RT-PCR, Golden Gate/Gibson assembly, and mutagenesis in phage and yeast display vectors
• Conduct high-throughput screening (HTS) of antibody display campaign outputs using ELISA and flow cytometry
• Prepare and perform quality control (QC) on NGS libraries
• Analyze and interpret experimental data, and clearly communicate findings in presentations
• Work on multiple projects simultaneously, ensuring timely delivery of potential therapeutic leads with appropriate guidance
• Document data in an electronic lab notebook in an organized manner
• Innovate and establish new strategies to address challenging targets
  
  

Requirements:

• PhD or equivalent in molecular biology or a related field
• Direct experience with phage or yeast display systems required
• Direct experience in protein engineering
• In-depth knowledge and hands-on experience with molecular biology techniques, including RT-PCR, PCR, overlap extension PCR, cloning, primer design, and in-silico DNA construct design
• Experience with flow cytometry
• Experience in NGS sample preparation and library generation
• Experience in cloning, expression, and purification of proteins/antibodies in bacterial and mammalian cells
• Willingness to learn and adopt new techniques
  
  

Additional desirable skills include:

• Experience with NGS-based workflows, NGS sequencing, and data analysis is a plus
• Design and implement high-throughput mammalian cell-based assays
• Mammalian cell culture experience is a plus
• Strong problem-solving skills
• Effective resource and time management skills are essential
• Proficient in Microsoft Word, Excel, and PowerPoint, and experienced in maintaining an electronic lab notebook (ELN)
• Ability to adapt well to rapidly changing goals and research directions
  
  

This is a full-time position in Boulder, CO with in-person work in our labs.

Compensation Range: $90,000- $115,000

As a member of our team, you will be eligible for comprehensive, competitive, flexible benefits that include health and dental insurance, disability and life insurance, 401k with company match, and bonus opportunities based on company success.

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Mosaic Biosciences, Inc. is a discovery and preclinical development company that partners with biopharmaceutical companies to discover novel protein and antibody therapeutics. Our experienced and dedicated research teams collaborate with our partners to design, manage, and execute research programs to rapidly advance programs, starting at any point from early discovery into preclinical development.

Mosaic teams provide comprehensive, end-to-end discovery services, including: program assessment and strategy development; program management; antibody discovery and optimization; protein production, purification, and characterization; protein engineering; biochemical and cellular assay development; formulation, stability and developability studies; bioanalytical (PK and PD) assay development; and candidate-validating pharmacology.

Based in Boulder, Colorado, we are a collaborative and talented team of scientists and entrepreneurs looking for dynamic, hardworking, creative thinkers who excel in a cutting-edge, science-driven, team environment.

At Mosaic, we believe in a diverse workplace that celebrates all backgrounds and promotes individuality. We are committed to creating an inclusive environment with the purpose of furthering scientific innovation and supporting our employees by offering equal opportunities for all.

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