Raqib Hasan

San Antonio, Texas, United States Contact Info
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About

Biotechnologist with an advanced degree, specializing in Upstream Process Devlopmemt for…

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Experience & Education

  • Scorpius Biomanufacturing, Inc.

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Volunteer Experience

  • Organizing secretary

    JBHSRP

    - Present 12 years 2 months

    Health

  • SWAPNOTTHAN Graphic

    General Memeber

    SWAPNOTTHAN

    - 3 years

    Children

    Swapnotthan a voluntary organization dedicated to teach underprivileged children in sylhet city, Bangladesh. Volunteers spend time to educate kindergarten and high school children and prepare them to compete in the real world.

Publications

  • In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

    American Journal of Plant Sciences

    In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a…

    In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% - 50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA. These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.

    Other authors
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  • Efficient Callus Initiation and Plantlet Regeneration of Citrus japonica Margarita

    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN:2278-3008, p-ISSN:2319-7676. Volume 11, Issue 3

    In vitro approaches has become necessary for overcoming the hurdles of cultivation of Citrus, a commercially important fruit. The present study deals with establishment of protocol for micro propagation of Citrus Japonica Margarita through callus induction and regeneration. Mature seeds of this variety of citrus are used as explant. The Explants were cultured on Murashige and Skoog medium (MS) containing 30 g/L sucrose and 7 g/L agar supplemented with different concentrations and combinations…

    In vitro approaches has become necessary for overcoming the hurdles of cultivation of Citrus, a commercially important fruit. The present study deals with establishment of protocol for micro propagation of Citrus Japonica Margarita through callus induction and regeneration. Mature seeds of this variety of citrus are used as explant. The Explants were cultured on Murashige and Skoog medium (MS) containing 30 g/L sucrose and 7 g/L agar supplemented with different concentrations and combinations of different phyto-hormones; 6-benzylaminopurine (BA), naphthalene acetic acid (NAA), 2,4-dichloro phenoxy acetic acid (2,4 D). The maximum callus induction (88%) was observed from the mature seeds of Citrus japonica observed on MS medium supplemented with 2,4 D 16 ìM. Maximum shoot regeneration response (70%) was observed on MS medium supplemented with BA 13.0 ìM. Maximum root regeneration response (80%) was observed on MS medium supplemented with IBA 10 ìM and when it is supplemented with NAA 5 ìM it is 70%. The regenerated plantlets were successfully acclimatized in pots containing sterile soil mixture to study their response in in vivo conditions.

    Other authors
    • Shahariar Mohammed Fahim
    • Md. Abdullah Shamim

  • In Vitro Propagation of an Endangered Wild Orange Species Citrus chrysocarpa L.

    International Journal of Sciences: Basic and Applied Research (IJSBAR) ISSN 2307-4531

    A method for in-vitro propagation of wild type Indian orange (Citrus chrysocarpa L.) was developed by shoot organogenesis from seed. Mature seed embryos were used as explants and treated with different hormones and plant growth regulators on MS medium for callus, shoots and roots induction. For callus induction seed embryos were treated with different concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and a-naphthalene acetic acid (NAA) and maximum 90.90% callus…

    A method for in-vitro propagation of wild type Indian orange (Citrus chrysocarpa L.) was developed by shoot organogenesis from seed. Mature seed embryos were used as explants and treated with different hormones and plant growth regulators on MS medium for callus, shoots and roots induction. For callus induction seed embryos were treated with different concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and a-naphthalene acetic acid (NAA) and maximum 90.90% callus was observed on MS medium supplemented with 2, 4 -D at 16.0 ìM concentration. Approximately 85% of the callus was granular, while 15% was smooth and compact. Maximum 83.33% shoot regeneration were observed on MS medium supplemented with BA at 13ìM concentration. Regenerated shoots were rooted on same medium supplemented with different hormones and maximum 80% were observed in MS medium supplemented with 10 ìM Indole-3-butyric acid (IBA). The regenerated plantlets were successfully acclimatized in ex-situ condition.

    Other authors
    • Md. Nazmul Hasan
    • Shamsul Haque Prodhan

  • Development of an Efficient in Vitro Regeneration System for Endangered Wild Orange Citrus Chrysocarpa L

    International Journal of Sciences: Basic and Applied Research (IJSBAR)

    A method for in-vitro propagation of wild type Indian orange (Citrus chrysocarpa L.) was developed by shoot organogenesis from seed. Mature seed embryos were used as explants and treated with different hormones and plant growth regulators on MS medium for callus, shoots and roots induction. For callus induction seed embryos were treated with different concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and a-naphthalene acetic acid (NAA) and maximum 90.90% callus…

    A method for in-vitro propagation of wild type Indian orange (Citrus chrysocarpa L.) was developed by shoot organogenesis from seed. Mature seed embryos were used as explants and treated with different hormones and plant growth regulators on MS medium for callus, shoots and roots induction. For callus induction seed embryos were treated with different concentration of 2, 4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BA) and a-naphthalene acetic acid (NAA) and maximum 90.90% callus was observed on MS medium supplemented with 2, 4 -D at 16.0 M concentration.

    Approximately 85% of the callus was granular, while 15% was smooth and compact. Maximum 83.33% shoot regeneration were observed on MS medium supplemented with BA at 13M concentration. Regenerated shoots were rooted on MS medium supplemented with different hormones and maximum 80% roots were observed in MS medium supplemented with 10 M Indole-3-butyric acid (IBA). The regenerated plantlets were successfully acclimatized in ex-situ condition for further study.

    Other authors
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  • Efficient Regeneration System for the Improvemen Kinnow mandarin (Citrus reticulata Blanco),

    Journal of Biology, Agriculture and Healthcare, Vol 6, No 4.

    Kinnow mandarin (Citrus reticulata Blanco.) is a highly adaptable variety among citrus cultivars. An efficient system for in vitro regeneration by organogenesis starting from seed of (C. reticulata Blanco) was developed. Seeds were treated by Murashige and Skoog (MS) media supplemented with 2, 4-Dichlorophenoxyacetic acid (2,4-D) to initiate callus induction. The best result (96%) were obtained when seeds were treated with MS basal media + 2,4-D (16.0) μM. The regeneration system tested allowed…

    Kinnow mandarin (Citrus reticulata Blanco.) is a highly adaptable variety among citrus cultivars. An efficient system for in vitro regeneration by organogenesis starting from seed of (C. reticulata Blanco) was developed. Seeds were treated by Murashige and Skoog (MS) media supplemented with 2, 4-Dichlorophenoxyacetic acid (2,4-D) to initiate callus induction. The best result (96%) were obtained when seeds were treated with MS basal media + 2,4-D (16.0) μM. The regeneration system tested allowed the attainment of highest shoots (90 %) with BA 13.0 μM. An average of 7.8 well-differentiated shoots per explant was obtained. Highest rooting (85%) was achieved in culture medium with 10.0 μM IBA. The well-developed plantlets were transferred to potting mixture. Of the rooted plant, 95% adapted well to soil conditions.

    Other authors
    • Anup Gupta
    • Md Nazmul Hasan

Projects

  • Genetic analysis of bacterial wilt pathogenesis through transposon mutagenesis of the pathogen, Erwinia tracheiphila.

    - Present

    To investigate the virulent factors of bacteria wilt pathogen Erwinia tracheiphila (Et) and develop molecular tools that can be used to successfully study Et at a genetic level.

  • Isolation and characterization of GFP-tagged transposon mutants of Pectobacterium carotovorum subsp. carotovorum KD100.

    -

  • Agrobacterium mediated Gene Transfer in Citrus reticulata Blanco

    -

    This project explored an improved system for efficient regeneration of Kinnow mandarin (Citrus reticulata Blanco) to improve crop yield and more abiotic stress tolerance.

Honors & Awards

  • Project proposal, Kaizen Project Fair

    -

  • Scientific poster, Annual Plant Tissue Culture and Biotechnology Conference.

    -

  • Scientific poster, Young Biotechnologist of Bangladesh (YBB), 3rd Congress.

    -

Languages

  • English

    -

  • Bangla

    -

Organizations

  • American Association for the Advancement of Science (AAAS).

    member

    - Present

  • American Society forMicrobiology (ASM).

    member

    - Present

  • Bangladesh Association of Biotechnology Graduate

    Member

    - Present

  • Bangladesh Association for Plant Tissue Culture and Biotechnology

    Member

    - Present

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