Allele-specific detection of single mRNA molecules in situ
CH Hansen, A Van Oudenaarden�- Nature methods, 2013 - nature.com
CH Hansen, A Van Oudenaarden
Nature methods, 2013•nature.comWe describe a method for fluorescence in situ identification of individual mRNA molecules,
allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts
that differ by only a few nucleotides. By using a combination of allele-specific and non–allele-
specific probe libraries, we achieve> 95% detection accuracy. We investigate the allele-
specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine
embryonic stem cells.
allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts
that differ by only a few nucleotides. By using a combination of allele-specific and non–allele-
specific probe libraries, we achieve> 95% detection accuracy. We investigate the allele-
specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine
embryonic stem cells.
Abstract
We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non–allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.
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