Visualizing SNVs to quantify allele-specific expression in single cells
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons
and introns on individual RNA transcripts with high efficiency. We used this method to
quantify allelic expression in cell populations and in single cells, and also to distinguish
maternal from paternal chromosomes in single cells.
and introns on individual RNA transcripts with high efficiency. We used this method to
quantify allelic expression in cell populations and in single cells, and also to distinguish
maternal from paternal chromosomes in single cells.
Abstract
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells.
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