[HTML][HTML] The role of 3′-5′ exonucleolytic proofreading and mismatch repair in yeast mitochondrial DNA error avoidance
S Vanderstraeten, S Van den Brûle, J Hu…�- Journal of Biological�…, 1998 - ASBMB
In the D171G/D230A mutant generated at conserved aspartate residues in the Exo1 and
Exo2 sites of the 3′-5′ exonuclease domain of the yeast mitochondrial DNA (mtDNA)
polymerase (pol-γ), the mitochondrial genome is unstable and the frequency of mtDNA point
mutations is 1500 times higher than in the wild-type strain and 10 times higher than in single
substitution mutants. The 10 4-fold decrease in the 3′-5′ exonuclease activity of the
purified mtDNA polymerase is associated with mismatch extension and high rates of base�…
Exo2 sites of the 3′-5′ exonuclease domain of the yeast mitochondrial DNA (mtDNA)
polymerase (pol-γ), the mitochondrial genome is unstable and the frequency of mtDNA point
mutations is 1500 times higher than in the wild-type strain and 10 times higher than in single
substitution mutants. The 10 4-fold decrease in the 3′-5′ exonuclease activity of the
purified mtDNA polymerase is associated with mismatch extension and high rates of base�…