[HTML][HTML] Rheb binding to mammalian target of rapamycin (mTOR) is regulated by amino acid sufficiency
X Long, S Ortiz-Vega, Y Lin, J Avruch�- Journal of Biological Chemistry, 2005 - ASBMB
The removal of extracellular amino acids or leucine alone inhibits the ability of the
mammalian target of rapamycin (mTOR) to signal to the raptor-dependent substrates, p70
S6 kinase and 4E-BP. This inhibition can be overcome by overexpression of the Rheb
GTPase. Rheb binds directly to the amino-terminal lobe of the mTOR catalytic domain, and
activates mTOR kinase in a GTP-dependent manner. Herein we show that the binding of
Rheb to endogenous and recombinant mTOR is reversibly inhibited by withdrawal of all�…
mammalian target of rapamycin (mTOR) to signal to the raptor-dependent substrates, p70
S6 kinase and 4E-BP. This inhibition can be overcome by overexpression of the Rheb
GTPase. Rheb binds directly to the amino-terminal lobe of the mTOR catalytic domain, and
activates mTOR kinase in a GTP-dependent manner. Herein we show that the binding of
Rheb to endogenous and recombinant mTOR is reversibly inhibited by withdrawal of all�…