Activation of Nrf2 by H2O2: de novo synthesis versus nuclear translocation
The most common mechanism described for the activation of the transcription factor Nrf2 is
based on the inhibition of its degradation in the cytosol followed by its translocation to the
nucleus. Recently, Nrf2 de novo synthesis was proposed as an additional mechanism for the
rapid upregulation of Nrf2 by hydrogen peroxide (H 2 O 2). Here, we describe a detailed
protocol, including solutions, pilot experiments, and experimental setups, which allows
exploring the role of H 2 O 2, delivered either as a bolus or as a steady state, in endogenous�…
based on the inhibition of its degradation in the cytosol followed by its translocation to the
nucleus. Recently, Nrf2 de novo synthesis was proposed as an additional mechanism for the
rapid upregulation of Nrf2 by hydrogen peroxide (H 2 O 2). Here, we describe a detailed
protocol, including solutions, pilot experiments, and experimental setups, which allows
exploring the role of H 2 O 2, delivered either as a bolus or as a steady state, in endogenous�…