Since the days of Sanger sequencing, next-generation sequencing technologies have
significantly evolved to provide increased data output, efficiencies, and applications. These
next generations of technologies can be categorized based on read length. This review
provides an overview of these technologies as two paradigms: short-read, or “second-
generation,” technologies, and long-read, or “third-generation,” technologies. Herein, short-
read sequencing approaches are represented by the most prevalent technologies, Illumina�…